中文摘要：

建立液-质联用法以同时测定人参制剂中人参皂苷Rg1、Rb1、Re、Rd的含量.色谱柱为Waters symmetry C18（150 mm×2.1 mm,3.5μm）,流动相为乙腈-甲醇-水（50∶20∶30,含0.1%甲酸）,流速为0.2 mL/min,柱温为30℃,采用正离子多反应监测模式检测.在本研究建立的实验条件下人参皂苷Rg1、Rb1、Re和Rd 4种成分线性关系良好,精密度、重复性、回收率的RSD均小于6%.本方法准确、灵敏、特异性好,流动相等度洗脱的条件下3.5 min之内便可完成4种皂苷的同时分离,适用于人参制剂中人参皂苷Rg1、Rb1、Re、Rd含量的同时测定.

英文摘要：

A sensitive and rapid liquid chromatography-tandem mass spectrometry method is developed to determine the content of ginsenoside Rg1, Rb1, Re, Rd in the ginseng preparation. The column of Waters symmetry C18 （ 150 mm× 2.1 mm, 3.5 μm） is employed for separation, the mobile phase is composed of 50% acatonitril, 20% methanol and 0.1% formic acid, and the flow rate is 0.2 mL/min. The data are acquired by the multiple reactions monitoring in the positive ion mode. In these chromatographic conditions, all the compounds show good linearity. The RSD of precision, repeatability and recovery in experiments are less than 6%. Four ginsenosides separation is achieved with the mobile phase isocratic elution in a total running time of 3.5 min. This method is proven to be specific, accurate, sensitive, and can be successfully applied to determine the contents of four ginsenoside in the ginseng preparations.