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中文摘要：
目的研究激活素受体相互作用蛋白2（ARIP2）在小鼠肝细胞中的表达及调控。方法采用半定量PCR技术检测ARIP2mRNA在Hepal-6细胞中转录水平的动力学变化规律及其调控因素。结果ActivinA刺激Hepal-6细胞ARIP2mRNA的转录水平呈时间依赖性升高，刺激早期（4h）无明显变化，12h后显著升高。信号传导激动剂PMA和LPS刺激He-pal-6细胞24h，均可上调ARIP2mRNA的转录水平，而A23187则抑制其转录。ARIP2过表达明显抑制Hepal-6细胞ActRIIAmRNA的转录水平，对ActRIIB则无影响。结论ARIP2作为激活素信号传导抑制蛋白，其表达受多种因素影响。ARIP2可能通过影响ActRIIA表达，参与激活素作用后期的信号传导负反馈调节过程。
英文摘要：
Objective To study the expression and regulation of activin receptor-interacting protein 2 （ARIP2） in mouse Hep- al-6 cells. Methods Determine the dynamics of transcription levels of ARIP2 mRNA in Hepal-6 cells by semi-quantitative PCR and explore the factors regulating the expression. Results Time -dependent increase of transcription level of ARIP2 mRNA was observed in Hepal-6 cells stimulated by activin A. The transcription level showed no significant change at early stage （4 h） but increased signifi- cantly 12 h after transcription. After Hepal-6 cells were stimulated with either signal transduction activator PMA or LPS for 24 h, the transcription level of ARIP2 mRNA increased significantly. However, A23187 inhibited the transcription. The overexpression of ARIP2 inhibited the transcription of ActRIIA mRNA in Hepal-6 cells significantly, but showed no significant influence on that of ActRIIB mR- NA. Conclusion The expression of ARIP2 as an inhibitor of activin signal transduction were influenced by various factors. ARIP2 might participate in the negative feedback regulation of signal transduction at late stage by affecting the expression of ActRIIA.
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