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Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1
  • ISSN号:0253-505X
  • 期刊名称:《海洋学报:英文版》
  • 时间:0
  • 分类:Q987[生物学—遗传学;生物学—人类学] S512.101[农业科学—作物学]
  • 作者机构:[1]Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China, [2]College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China, [3]Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
  • 相关基金:The Key Natural Science Foundation of Fujian under contract No. 2007J0004; the National Natural Science Foundation of China under contract No. 40576076 Acknowledgements The authors thank Dr. Zhou Fengfeng from Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology and Institute of Bioinformatics, University of Georgia for his kind help in gene analysis.
中文摘要:

thermophiles 的病毒由于他们在基因转移,全球 geochemical 周期和在地球上的生活的进化的角色兴趣大。然而, thermophilic 抗菌素没广泛地被学习。在这调查,典型抗菌素 BV1 从 thermophilic 细菌 Geobacillus sp 被获得。6k512,它在中国的厦门从一个沿海的温泉被孤立。包含的 BV1 35 055 bp 的线性 DNA,它编码 54 开的读物装裱的双 stranded (ORF ) 。有趣地, ORF 分享了的 54 BV1 中的八个从人的疾病相关的细菌定序类似到基因。净化的 BV1 virions 的七蛋白质被 proteomic 分析识别。BV1 功能的 genomics 的决心将为 virus-thermophile 相互作用便于机制的更好的理解。

英文摘要:

Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames (ORFs). Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.

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期刊信息
  • 《海洋学报:英文版》
  • 主管单位:
  • 主办单位:中国海洋学会
  • 主编:潘德炉
  • 地址:北京海淀大慧寺路8号
  • 邮编:100081
  • 邮箱:hyxbe@263.net
  • 电话:010-62179976
  • 国际标准刊号:ISSN:0253-505X
  • 国内统一刊号:ISSN:11-2056/P
  • 邮发代号:82-285
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国剑桥科学文摘,美国科学引文索引(扩展库),英国科学文摘数据库,英国动物学记录
  • 被引量:331