中文摘要：

目的研究PTD-OD-HA融合蛋白对BCR-ABL阳性细胞株增殖的影响。方法将前期制备的PTD-OD-HA融合蛋白作用于两种BCR-ABL阳性细胞株BaF3-P210和K562,同时设立PBS和OD-HA融合蛋白对照组,用MTT法检测细胞的生长情况。48h后离心收集细胞,用瑞氏染色及透射电镜观察细胞形态改变,流式细胞仪（FCM）检测细胞周期变化,RT-PCR、Westernblot-ting检测c-myc、cyclinD1mRNA及蛋白表达的变化。结果 MTT检测发现,与PBS和OD-HA对照组比较,PTD-OD-HA能抑制BCR-ABL阳性细胞株的生长。瑞氏染色及透射电镜观察见PTD-OD-HA处理的细胞胞质内有大量空泡样变,出现大量的溶酶体,线粒体广泛扩张;FCM检测发现G1期细胞比例增加,S期和G2期细胞比例减少;RT-PCR及Westernblotting检测发现c-myc、cyclinD1的mRNA及蛋白水平均降低。结论 PTD-OD-HA融合蛋白可抑制BCR-ABL阳性细胞生长,使细胞形态发生改变,并使细胞阻滞在G1期,这些改变可能是通过下调c-myc、cyclinD1的表达实现的。

英文摘要：

Objective To study the effects of PTD-OD-HA fusion protein on the proliferation of BCR/ABL positive cell lines.Methods PTD-OD-HA fusion protein had been purified formerly.The BCR/ABL positive cell lines BaF3-P210 and K562 were then treated with PTD-OD-HA fusion protein,and PBS and OD-HA fusion protein treated groups were set as control at the same time.The cell growth was detected by MTT test.Forty-eight hours after treatment,cells were collected,the cell morphology was observed with Wright′s stain and transmission electron microscopy （TEM）,the cell cycles were determined by flow cytometry （FCM）,and the levels of c-myc and cyclin D1 were determined by RT-PCR and Western blotting.Results MTT test revealed that,compared to PBS and OD-HA fusion protein treated groups,PTD-OD-HA fusion protein inhibited the growth of BCR/ABL positive cells;a large number of vacuoles were found in the cytoplasm after PTD-OD-HA protein treatment,and lysosome increased significantly and mitochondria expanded extensively in BaF3-P210 cells.FCM showed that cells of G_1 phase increased,while of S phase and G_2 phase decreased.RT-PCR and Western blotting revealed that the mRNA and protein levels of c-myc and cyclin D1 decreased in BaF3-P210 and K562 cells.Conclusions PTD-OD-HA fusion protein can inhibit the growth of BCR/ABL positive cells,induce cell morphological changes,and the cell cycle may be blocked in G1 phase.These changes may be caused by down-regulation of the expression of c-myc and cyclin D1.