本文采用药膜法建立了朱砂叶螨Tetranychus cinnabarinus(Boisduval)对5种杀螨剂的敏感基线,并对6个不同地理种群的朱砂叶螨进行了抗药性监测,结果表明:5种药剂杀螨活性由高到低分别为阿维菌素〉丁氟螨酯〉氧化乐果〉炔螨特〉甲氰菊酯,其对朱砂叶螨雌成螨的LC50值分别为0.08、2.19、67.89、201.19和605.27mg/L;朱砂叶螨各地理种群已对甲氰菊酯和炔螨特产生了低、中水平的抗性,其抗性倍数分别介于2.93~16.22与4.85~14.35之间,其中云南种群对这2种杀螨剂抗性最高,对氧化乐果与丁氟螨酯处于敏感性降低阶段,其抗性倍数分别介于2.35~4.26与1.56~2.11之间,对阿维菌素还未产生明显抗性;对阿维菌素和甲氰菊酯的增效剂生物测定结果表明,三类解毒酶系(多功能氧化酶、谷胱甘肽S-转移酶和酯酶)都不同程度地参与了朱砂叶螨抗药性的形成。
The baseline sensitivity of 5 acaricides against Tetranychus cinnabarinus(Boisduval) was established and the resistance level of 6 different field strains of T.cinnabarinus to these acaricides monitored using the residual contact vial method.The results show that the relative toxicity of the 5 acaricides to T.cinnabarinus was,abamectincyflumetofenomethoatepropargitefenpropathrin.The LC50 of female adults were 0.080 mg/L,2.19 mg/L,67.89 mg/L,201.19 mg/L and 605.27 mg/L,respectively.The 6 field strains developed 2.93-16.22 fold resistance to fenpropathrin and 4.85-14.35 fold resistance to propargite.Of the 6 strains tested,the Yunnan strain showed the highest resistance to both acaricides.The six field strains displayed lower sensitivity to cyflumetofen and omethoate,developing 1.56-2.11 and 2.35-4.26 fold resistance,to these pesticides respectively.No strains developed significant resistance to abamectin.The effects of synergists in susceptible and resistant strains (abamectin-resistant and fenpropathrin-resistant strains) were tested.The results show that three detoxification enzymes (PBO,GSTs and Esterases) contributed,to different degrees,to the resistance of T.cinnabarinus to acaricides.