中文摘要：

目的：研究甘肃保安族口腔健康儿童唾液微生物群落结构,探寻优势微生物种群。方法：通过构建16S rRNA克隆文库法对测序结果进行分析,利用BLAST、Mothur、Clustalx 2.0等软件进行细菌群落结构多样性分析,构建系统发育树。结果：共检测到84个操作分类单位（OTU）,归属于5个门,12个纲,15个目,18个科,19个属。其中以厚壁菌门（Firmicutes,88.60%）比例最多;6个优势菌属（检出率〉1%）,分别为链球菌属（Streptococcus,70.28%）、颗粒链菌属（Granu Iicatella,6.20%）、孪生菌属（Gemella,3.10%）、韦永氏球菌属（Veillonella,1.81%）、奈瑟菌属（Neisseria,1.03%）和嗜血杆菌属（Haemophilus,1.03%）,其中链球菌属在8个样本中均出现。结论：保安族口腔健康儿童唾液微生物群落结构较为丰富;链球菌属为主要优势菌。

英文摘要：

AIM：To explore the dominant salivary microbiota in healthy children of Bonan ethnic group of Gansu province.METHODS：16S rRNA clone library was constructed to analyze the sequencing results.The analysis of diversity of bacterial community structure was conducted by BLAST,Mothur and Clustalx2.0.Phylogenetic tree was constructed.RESULTS：84 operational takonomic units（OTUs）were detected which belonged to 5 phyla,12 classes, 15 orders,18 families and 19 genera.Firmicutes was the most prominent （88.60%）species.6 genera were at high a-bundance （detection rate 〉1%）：Streptococcus （70.28%）,GranuIicatella （6.20%）,Gemella（3.10%）,Veillonel-la（1.81%）,Neisseria（1.03%）and Haemophilus（1.03%）.Streptococcus was detected in all 8 samples.CONCLU-SION：The salivary microbial community structure of healthy children of Bonan ethnic group is abundant.Streptococcus is the dominant bacteria.