中文摘要：

通过检测类风湿性关节炎（rheumatoid arthritis，RA）患者外周嗜碱性粒细胞的活化情况，分析其活化与RA发病的关系，进一步阐明RA的发病机制。流式细胞术检测正常对照组和RA患者组外周嗜碱性粒细胞的活化情况及其表达炎症因子和归巢受体的情况。结果显示，RA患者外周嗜碱性粒细胞表达活化标志物CD203c的水平显著高于正常对照组（P〈0．05）。RA患者外周表达IL-4、IL-6和IL-13的嗜碱性粒细胞比例以及CD62L和CCR7表达水平均显著高于正常对照组（均P〈0．05）。RA患者外周嗜碱性粒细胞处于高度活化状态，并表达高水平的炎症因子和归巢受体，提示其活化后可能趋化至局部，进而参与RA的发病。

英文摘要：

We set out this study to investigate the activation of peripheral basophils of rheumatoid arthritis（RA） patients and analyze its relationship with the pathogenesis of RA. The activation of peripheral basophils and expression of inflammatory cytokines and lymph node homing receptors, by basophils from RA patients and healthy controls were detected by flow cytometry. The results showed that the expression of CD203c by basophils from RA patients were higher than that of healthy controls （P〈0, 05）. The proportion of IL-4, IL-6 and IL-13 positive basophils, and expression levels of CD62L and CCR7 on hasophils from RA patients were significantly higher than those of the healthy controls（all P〈0. 05）. In conclusion, basophils are activated in RA patients with high expression of inflammatory cytokines and lymph node homing receptors, thus provides the possibility for basophils to migrate into local sites and maybe involved in the pathogenesis of RA.