中文摘要：

目的 通过激活小鼠肝脏X受体，观察胆汁脂质成分变化与胆固醇代谢相关基因的表达情况，观察肝脏X受体在胆石病发生中的作用。方法 C57BL小鼠18只，分为2组，分别管饲肝脏X受体激动剂T0901317和安慰剂。分析胆汁脂质成分。实时定量PCR法测定肝脏与腹腔巨噬细胞的胆固醇代谢相关基因mRNA表达量。结果 实验组胆汁胆固醇摩尔百分比较对照组升高89％。实验组肝脏组织Abcg5、Abcg8、Abcal与Srebpl表达显著高于对照组：Abc茚为2．96±0．55比1．10±0．22（P〈0．01）；Abcg8为0．86±0．16比0．29±0．07（P〈0．01）；Abcal为8．45±1．06±5．51±0．84（P〈0．05）；Srebpl为14．08±3．18比3．06±0．74（P〈0．01）。腹腔巨噬细胞Abcal表达实验组高于对照组（27．66±7．18比8．39±3．65，P〈0．05）。结论 肝脏X受体激活使胆固醇逆转运增强，肝脏摄取的过多胆固醇通过胆汁清除，导致胆汁胆固醇含量升高，提示肝脏X受体在胆石病发生中有重要作用。

英文摘要：

Objective To study the changes of the biliary lipids composition and the expression of genes related to cholesterol metabolism in mice upon activation of liver X receptors. Methods Eighteen C57BL mice were divided into experimental group and control group randomly. Experimental group received T0901317 by gavage for 5 days. And control group was treated with the solvent only. Biliary lipids composition was measured. The mRNA expression levels of genes in liver and macrophages were analyzed by real-time PCR. Results The molar percent of biliary cholesterol was increased by 89% in experimental group as compared with control group. T0901317 treatment elevated the expression levels of Abcg5, Abcg8,Abcal and Srebpl significantly in liver （experimental group vs control： AbcgS,2.96 ± 0.55 vs 1.10±0.22,P 〈0.01 ; AbcgS,0.86 ±0.16 vs 0.29 ±0.07,P 〈0.01; Abcal,8.45 ± 1.06 vs 5.51 ± 0.84,P 〈 0.05 ; Srebpl, 14.08 ± 3. 18 vs 3.06 ± 0.74, P 〈 0.01 ）. The expression levels of Abcal in macrophages were increased significantly in experimental group as compared with control group （27.66 ± 7.18 vs 8.39 ± 3.65,P 〈 0.05）. Conclusion Reverse cholesterol transport was enhanced from peripheral ceils to liver upon activation of liver X receptors. Excess cholesterol in liver was eliminated by secretion into bile. These processes caused elevation of cholesterol content in bile. The results highlight the role of liver X receptors in the pathogenesis of gallstone formation.