中文摘要：

用石油醚、乙酸乙酯、氯仿和二次蒸馏水分别提取四物汤药剂,得到不同极性部位的溶剂提取物。促进骨髓间充质干细胞（rMSCs）增殖活性用四甲基偶氮唑盐（MTT）法,结果表明,四物汤乙酸乙酯提取部位（A-2）具有促进MSCs增殖的活性。用硅胶柱层析的方式对A-2作梯度洗脱得到20个组分,记为F-1-F-20。经MTT法和流式细胞技术评价,选择具有促进MSCs增殖的活性的F-4、F-7、F-10和F-11组分。采用HPLC、红外光谱和质谱检测发现,组分F-4为藁本内酯;用HPLC-MSn发现,F-11中含量达84.47%的成分为6,7-二羟基-烯丙基苯酞。HPLC结果显示,F-7和F-10有较大含量的脂肪酸酯类化合物。选用4个酯类标准品,先用MTT法和流式细胞技术对其评价;结果显示浓度为90mg/L时,十六酸甲酯（S-1）和十八酸乙酯（S-4）的增殖指数（PI）均高于对照组,具有促进MSCs体外增殖作用;而十六酸乙酯（S-2）和十八酸甲酯（S-3）增殖指数（PI）均低于对照组,具有抑制MSCs体外增殖作用。对F-7鉴定中检出含有S-1（3.04%）,S-2（3.92%）,S-4（9.61%）,在F-10中检出S-1（14.46%）,S-2（17.47%）,S-3（3.19%）,S-4（1.03%）。生物活性显示F-7活性强于F-10。初步推测在酯类混合物中,对MSCs起促进作用主要为S-1和S-4。结果表明,四物汤乙酸乙酯提取部位具有促进MSCs增殖作用的成分是藁本内酯、十六酸甲酯和十八酸乙酯。

英文摘要：

The Si-wu decoction （SDE） was extracted by petroleum ether, ethyl acetate, chloroform and redistillation water in order to gain different polar extractors. The cytoactive of extractions was carry out by 3-（4,5- dimethyl-thiazol-2-yl） -2,5-diphenyhetrazolium bromide （MTT） assay. The results demonstrated ethyl acetate extraction （A-2） might induce proliferation of marrow mesenchymal stem ceils （MSCs） in vitro. As a consequence, the further separation, composition analysis and activity experiments were mainly concerned to A-2. The analytical techniques such as GC-MS, HPLC and HPLC-MSn were also been used in constituent analysis. As regards to bioactivity analysis, MTT method and flow cytometry were mutual corroboration. The gradient elution by column chromatography was introduced to separate A-2, and twenty samples were marked with F-1 -F-20. Ligustilide （F-4） isolated from SDE was showed the best proliferation-promoting effect which detected by HPLC, IR spectrum and mass spectrum. 6,7-dihydroxy-3-（-prop-l-enyl） isobenzofuran-1 （3H） -one, identified by HPLC-MSn whose structure was similar with ligustilide, main constituent in F-11, the relative peak area was 84.47%. MTT assay manifested F-11 ＇s bioactivity was merely inferior to ligustilide. The quali- tative and quantitative analysis demonstrated there were large content of fatty esters in F-7 and F-10. Four fatty esters were chosen as standards to evaluate the bioactivity by flow cytometry. The proliferation index （PI） of palmitic acid methyl ester （S-1） and stearic acid ethyl ester （S-4） were higher than control group, which showed proliferation on MSCs, while palmitic acid ethyl ester （S-2） and stearic acid methyl ester （S-3） were lower than control group, which showed inhibition. The quantitative analysis on F-7 manifested that S-1 （3.04%） ,S-2 （ 3.92% ）, S-4 （ 9. 61% ）, while F-10 showed that S-1 （ 14. 46% ）, S-2 （ 17. 47% ）, S-3 （3. 19% ） ,S-4（ 1.03% ）. Bioactivity suggested th