中文摘要：

目的：观察电针对吲哚菁绿（ICG）在肝脏代谢的影响，探讨针灸对肝脏功能调节的机制，同时探讨活体荧光成像技术用于针灸机制研究的可行性。方法：将40只昆明种小鼠随机分为电针组和对照组，每组20只，结合经典的吲哚菁绿肝脏代谢试验，对尾静脉注射IcG后的20只小鼠电针“足三里”穴20min，用动物活体荧光成像仪观察电针20min和停针60min内ICG荧光在肝脏的强度和分布；20只小鼠作为对照组，只注射ICG不予以电针。比较两组荧光强度及分布区域的差异。结果：（1）在ICG注射即刻，两组小鼠肝脏即有团状聚集的荧光显示，随着观察时间的推移，荧光显示亮度和面积增加，至注射30min左右，荧光亮度减弱，该过程与已知的ICG在肝脏代谢活动是一致的。（2）电针组在电针10、20min和停针后，肝脏的荧光强度较对照组均减弱，荧光的分布面积也比对照组小。（3）对光强度值定量统计显示，在观察全程的各时间点，对照组该值较高，电针组则较小，并始终规律地出现。对所有观察时点ICG荧光强度均差值总和的统计显示，对照组和电针组之间具有统计学差异（P〈0．05）。结论：电针减弱ICG荧光强度和持续时间，说明电针加速了ICG在肝脏的代谢过程。动物活体荧光成像技术用于针灸作用原理研究是可行的。

英文摘要：

Objective To observe the influence of electroacupuncture （EA） on indocyanine green（ICG）metabolism in the liver and the action mechanism of acupuncture for hepatic regulation . Methods Forty Kunming mice were randomly divided into an EA group and a control group, 20 cases in each one. Combined with classical hepatic metabolism test of ICG, after tail vein injection of ICG, twenty mice were treated with EA at ＂Zusanli＂（ST 36） for 20 min. The ICG fluorescence intensity and distribution in the liver were observed with animal in vivo fluorescence imaging apparatus during 20 rain of EA and 60 rain of needle withdrawal. Twenty mice, taken as control group, were treated with ICG injection and no EA. The differences of fluorescence intensity and distribution in both groups were compared. Results （1） There was blocking and gathered fluorescent sign in both groups when ICG was injected immediately. With the extension of observation time, the fluorescence brightness and area were increased until 30 min of injection. This was in accordance with known metabolism activities of ICG in the liver. （2） At 10 rain and 20 min of EA and after needle withdrawal, compared with the control group, the fluorescence intensity in the liver of the EA group was weaker and the distribution area was smaller. （3） According to quantitative statistics of fluorescence intensity, at each time point of treatment, the value in the control group was higher than that in the EA group, which appeared regularly all along. As for the total mean difference of ICG fluorescence intensity at all time points, there was statistical difference between the two groups （P;0.05）. Conclusion EA could reduce fluorescence intensity and lasting time of ICG, indicating that EA accelerates metabolic process of ICG in the liver. Also it is feasible to apply animal in vivo fluorescence imaging technology to the principle research of acupuncture effect.