中文摘要：

以3个新疆杏品种的叶片为试材,以其叶片基因组DNA为模板,分别对PCR扩增体系中的10×Buffer（含Mg2＋）、dNTP、上下游引物、TaqDNA聚合酶和退火温度5个因素设计6个梯度,研究了新疆杏品种自交不亲和S-RNase基因PCR扩增条件,建立其优化的PCR扩增体系。研究结果表明：其扩增程序为94℃预变性5min,94℃变性45s,52℃退火45s,72℃延伸2min,35个循环后72℃延伸10min,25μL反应体系10×Buffer（含Mg2＋）2.5μL,dNTP0.08mmol/L,上下引物0.16μmol/L,DNA80ng,DNA聚合酶1μL,此为最优的反应体系。

英文摘要：

In this experiment,the leaves of three Xinjiang apricot cultivas were used as test materials,and their DNAs were used as templates,6 levels of 10×buffer,primer,dNTP mixture,DNA polymerase and annealing temperature were set in this experimentation to study the PCR amplification conditions of self-incompatibility S-RNase gene in Xinjiang apricost and establish optimal PCR ampilification system.The results showed that the amplification program was 94 ℃ predegradation for 5 min,94 ℃ degradation for 45 s,52 ℃ anneal for 45 s,72 ℃ extension for 2 min,after 35 cycles 72 ℃ extension for 10 min;the 25 μL reaction mixture contained 10×Buffer （include Mg2＋） 2.5 μL,dNTP mixture 0.08 mmol/L,each primer 0.16 μmol/L,DNA 80 ng,Taq DNA polymerase 1 μL,which is the optimum system.