中文摘要：

为研究硫代葡萄糖苷（Glucosinolate，简称硫苷）对辐射损伤小鼠的保护作用，预先给小鼠口服不同剂量的硫苷粗提物，两周后给予2Gy^12C^6＋离子束全身均匀辐照。辐照后8h内用流式细胞仪检测受辐照小鼠脑、肝、肺细胞在各细胞周期的百分率，用彗星电泳技术检测受辐射小鼠脑、肝、肺细胞的拖尾率和拖尾长度。与单纯辐照未给药组相比，口服较低剂量的硫苷粗提物，可以使^12C^6＋离子束全身辐照后小鼠的脑、肝、肺细胞中G0／G1期细胞百分比显著降低（p〈0．05），G2／M期和S期的细胞百分比明显上升（p〈0．05），受辐照损伤细胞的拖尾率和拖尾长度也显著降低（p〈0．05），而在中、高剂量组间差异都不显著，无统计学意义。研究表明，较低剂量的硫苷能够降低^12C^6＋离子束辐照时对小鼠脑、肝、肺细胞的损伤，说明硫苷对小鼠辐射损伤具有一定的保护作用。

英文摘要：

In order to investigate the protective effects of glucosinolate on the mice induced by ^12C^6＋ ion beams the mice were fed orally with crude extracts of glucosinolate in various doses for two weeks, and then were whole-body irradiated with 2Gy ^12C^6＋ ions. At 8h after irradiation the cell cycle status of the cells obtained from mice brain, lung and liver were measured by flow cytometry. The frequencies of cells with tail moment and the tail lengths were determined by the single cell gel electrophoresis. Compared with irradiation only group the percentages of cells in G0/G1phase decreased statistically significantly, whereas the percentages in G2/M and S phases increased （p〈0.05） in groups irradiated and treated with glucosinolate in lower doses. Similarly, the frequencies of cells with tail moment and the tail lengths induced by ^12C^6＋ ion irradiation also decreased in brain, lung and liver cells （P〈0.05）. Nevertheless, no significant differences were observed in medium and higher doses groups irradiated and treated with glucosinolate by contrast with irradiation only group. The results show that the glucosinolate of lower doses can reduce the irradiation damages of mice induced by ^12C^6＋ ion beams, and related protecting mechanism needs further exploration.