中文摘要：

目的：初步探讨心脉隆注射液对新生SD大鼠海马神经干细胞增殖分化作用的影响。方法分离新生SD大鼠海马组织，采用无血清及单克隆培养方式获得大量神经干细胞，将不同浓度心脉隆注射液加入第3代神经干细胞单细胞悬液中有血清贴壁培养并分实验组和对照组，应用免疫荧光及细胞化学染色检测Nestin、5．溴脱氧尿嘧啶核苷（BrdU）、微管相关蛋白2（MAP-2）、胶质纤维酸性蛋白（GFAP）的表达，计算MAP一2阳性细胞百分率。四甲基偶氮唑盐（Mar）比色法观察心脉隆注射液对神经干细胞增殖能力的影响。结果海马NSCs在分化后，各心脉隆注射液干预组MAP一2阳性细胞比例均较空白对照组增高（P〈0．05），MTr结果显示培养5d后各药物组NSCs增殖能力均较对照组增强（P〈0．05）。结论心脉隆注射液具有促进神经干细胞增殖并向神经元方向分化的作用。

英文摘要：

Abstract： Objective To investigate the effects of xinmailong injection on the proliferation and differentiation of neural stem cells from the hippocampus of neonatal Sprague Dawley rats. Methods The hippocampal tissues of the newborn rat were dissociated and cultured with serum-free medium using single-cell cloning technique. Hippocampal neural stem cells of the 3rd passage were induced by xinmallong injection with various concentrations of serum and divided into the various experimental groups and the control group. Nestin, 5-bromo-2-deoxyuricine （BrdU）, microtubule-associated protein 2 （MAP-2）, and glial fibrillary acidicprotein （GFAP） expression were detected using immunofluorescence cyto- chemical staining. The percentages of MAP-2 positive cells were obtained. Monotetrazolium （MTT） assay was used to detect neural stem cell （NSCs） proliferation under the condition of xinmallong injection. Results The percentages of MAP-2 positive cells in the various experimental groups were higher（P 〈 0.05 ） than the control group. After 5 days culti- vation, the NSCs growth tendency in the experimental groups was increased compared to the control group. Conclusion Xinmailong injection may facilitate the proliferation of NSCs and promote the differentiation of NSCs into neurons.