中文摘要：

目的建立一种新型高效的中药材中低含量活性成分的富集方法。方法以白术内酯Ⅲ为模板分子、1一乙烯基咪唑为功能单体，采用沉淀聚合法制备了白术内酯Ⅲ分子印迹聚合物（MIPs），再将其作为固相萃取柱（SPE）填料对中药白术和党参药材提取液中白术内酯Ⅲ及其结构相近的活性成分进行富集分离。结果MIP—SPE和C18-SPE的富集因子（EF）分别为78．90和51．56μg·g-1，MIP对目标成分的选择性富集性能强于C18吸附填料。白术内酯Ⅲ分子印迹固相萃取法加样回收率为102．2％，检测限为0．36μg·mL-1。结论分子印迹固相萃取法可以作为定向富集低含量活性成分的有效方法。

英文摘要：

OBJECTIVE To establish a novel and efficient method for enriching low content active components from traditional Chinese herbs. METHODS The enrichment of atractylenolide Ⅲ was taken as an example. First, molecular imprinting polymers （MIPs） of atraetylenolide Ⅲ were prepared by precipitation polymerization using 1-Vinylimidazole as functional monomer. Second, the atractylenolide Ⅲ MIPs were packed into solid-phase column to separate atractylenolide Ⅲ and its analogues from the exracts of Herba Atractylodes Macrocephaia and Codonopsis pilosula. Moreover, the adsorption performance of MIPs for the target components was also investigated. RESULTS Enrichment factor （EF） of MIP-SPE and C ls-SPE column were 78.90 and 51.56 μg g- 1 respectively, suggesting that MIPs had better adsorption property than C18 ;the precision and accuracy of the developed method were satisfactory with recoveries of 102. 2% and LOD of 0. 36 μg mL-1. CONCLUSION These results demonstrate the feasibility of molecularly imprin- ted solid phase extraction for enriching low content active components in traditional Chinese herbs.