中文摘要：

海湾扇贝（Argopecten irradians）是雌雄同体型贝类，一般情况下每个个体的雌雄配子可在较短时间内相继排放，并且海湾扇贝没有自交不亲和现象，因此在群体繁殖过程中自体受精与异体受精可同时发生。这种特殊的生殖方式给海湾扇贝不同个体或群体之间的人工授精带来了困难，并因此阻碍了相关遗传学研究工作的开展。我们从2001年始开展海湾扇贝不同个体间杂交技术的研究，通过人为调控配子排放方式等多种措施实现了海湾扇贝群体间或个体间的人工授精。

英文摘要：

Hybrid family of Argopecten irradians irradians was created by fertilization between two nortbem bay scallop individuals. Two families were analyzed in this study. The first family, Pa - Pb, is a pair mating between two scallops named Pa and Pb, while the second one crossed by individuals of Y1 and P0. Marker inheritance and segregation were studied in the 10 progenies of each family by randomly amplified polymorphic DNA （RAPD） analysis. 102 RAPD primers were fast screened by parental animals of both families. Only the primers with polymorphisms between the two parental animals in each family were selected for further analysis. In both families, parents and 10 progeny were analyzed with selected primers. In family Pa - Pb, a total of 122 bands generated from 12 selected primers. 37 of them were polymorphic between two parents. The maternal Pa of this family had 17 molecular markers while paternal Pb bad 20 markers. In Y1 - P0,95 bands were produced by 10 selected primers. 32 bands were polymorphic between maternal Y1 and paternal P0, who had 17 and 15 molecular markers respectively. In both families, each progeny analyzed in this study had at least 8 maternal markers and 5 paternal markers. Based on segregation patterns at all markers analyzed, we concluded that none of the progeny analyzed were from self-fertilization, and one-way hybridization between two individuals was successful in both of the two bay scallop families.