中文摘要：

近似生理条件下，采用共振光散射法研究了CdSe/ZnS量子点/TiO2纳米复合物同人血清白蛋白的相互作用，通过分析影响二者相互作用的纳米复合物浓度、pH、NaCl浓度、反应温度、检测时间、共存离子、表面活性剂、加样顺序等外源刺激因素，结果表明：新形成的复合体系可能加强蛋白质的疏水腔，使在水溶液中形成的疏水界面趋于集中，从而导致其共振光散射强度增强；体系受pH的影响变化非常灵敏；适当的NaCl浓度可以提高体系的 IRLS值灵敏度；共存离子改变了体系的离子强度，从而导致体系的ΔIRLS值的改变；反应时间为5 min时，体系 IRLS值基本稳定；同一种表面活性剂对于不同的体系的 IRLS值的作用不完全一致，带相反电荷的表面活性剂与纳米复合物有很强的静电作用；对于与 HSA相互作用的多元复合体系，加样顺序的不同对体系的 IRLS值的影响很明显；体系的 IRLS值随温度的改变呈不完全单调增加的趋势。这些信息为纳米材料与生物大分子的相互作用机制提供理论支撑，有助于深入了解纳米材料的生物相容性和安全性。

英文摘要：

The interaction between CdSe/ZnS（quantum dots）/TiO2 nanocomposites and human serum albumin（HSA） was inves-tigated by resonance light scattering （RLS） spectroscopic methods under approximate physiological conditions .Much important information of the interaction between CdSe/ZnS（Quantum Dots）/TiO2 nanocomposites and HSA was obtained by studying com-prehensively the Exogenous influence factors of nanocomposites concentration ,pH ,NaCl concentration ,reaction temperature , detection time ,coexisting ions ,surfactants ,sequence of adding to the sample etc .It was showed that the new formation of com-plex system is likely to enhance the protein hydrophobic cavity and tend to focus the hydrophobic interface in aqueous solution , resulting in strengthening the intensity of resonance light scattering ;Also it is very sensitive to the changes in the pH value in the system ;The sensitivity of IRLS in system can be increased by the appropriate NaCl concentration ;The value of IRLS in sys-tem would be changed with the change in the concentration of coexisting ions ;The value of IRLS in system is basically stable when the reaction time reaches 5 min;The value of IRLS in system is not exactly the same with a surfactant ,and strong electrostatic in-teraction has occurred between oppositely charged surfactant and nano composites ;It is obvious that the value of IRLS in complex system is affected by the sequence of adding to sample ;It has the incomplete monotonically increasing trend with the changes in temperature .The information is useful for providing theoretical supporting for the mechanism of interaction between nanomateri-als and bio-macromolecule ,and for understanding the biocompatibility and safety of nano-materials .