中文摘要：

目的：探讨炎症抑制因子白细胞介素-10（白介素-10）在β-防御素2（hBD-2）基因诱导性表达中的作用。方法：22例健康人被纳入本研究中，以终浓度0ng／ml的LPS、100ng／ml的LPS、10ng／ml的IL-10、100ng／ml的LPS＋10ng／ml的IL-10加入900μl人外周血中，于37℃刺激培养6h后，提取外周血白细胞总RNA，用实时定量RT-PCR的方法测定外周血白细胞hBD-2mRNA的表达水平。结果：0ng／ml的LPS或10ng／ml的IL-10刺激6h的白细胞中未检测到hBD-2mRNA，100ng／ml的LPS刺激6h后hBD-2mRNA的表达水平为166．9±35．14，而IL-10与LPS共同刺激6h后hBD-2mRNA的表达水平为30．40±9．18。IL-10使hBD-2mRNA的诱导性表达水平明显降低（P〈0．05）。结论：人外周血白细胞中hBD-2基因呈现诱导性表达，IL-10能下调hBD-2的诱导性表达。

英文摘要：

Objective： To investigate the effect of IL-10 on the inducibility of human β-defensin 2 （hBD-2） in human peripheral blood cells. Methods： Peripheral blood samples were collected from 22 healthy individuals and co-cultured with 0 ng/ml lipopolysaccharide （LPS）, 100 ng/ml LPS, 10 ng/ml IL-10, or 100 ng/ml LPS plus 10 ng/ml IL-10 at 37℃ for 6 h. Total RNA was extracted from peripheral blood cells and the mRNA level of hBD-2 was detected by relative quantitative real-time PCR. Results： No detectable level of hBD-2 mRNA was found in normal peripheral blood cells with stimulation of 0 ng/ml LPS or 10 ng/ml IL-10. The mRNA level of hBD-2 was increased to 166.9±35.14 after 100 ng/ml LPS challenge, while the mRNA level of hBD-2 was decreased to 30.40±9.18 after the co-stimulation of 100 ng/ml LPS plus 10 ng/ml IL-10; which was significantly lower than that with LPS alone （P〈0.05）. Conclusion： The expression of hBD-2 gene can be induced by LPS. IL-10 inhibits the inducible expression of hBD-2.