中文摘要：

揭示在氧气的 anoxygenic phototrophic 细菌( AAnPB )和他们的亲戚，厌氧的 anoxygenic phototrophic 细菌( AnAnPB )和 nonphototrophic 细菌( NPB ，它与 AAnPB 有 16S rDNA 基因的高相同并且掉进一样的类)之间的种系发生、进化的关系的学习目的，并且验证 farnesyl 焦磷酸盐 synthase ( FPPS )的可靠性和实用性为种系发生的决心的基因。有我们的修改教材的 FPPS 基因和有一般教材的 16S rDNA 基因，被放大并且从 GenBank 定序或检索数据库。与 16S rDNA 基因相对照种系发生的树， AAnPB 没有在在 FPPS 上构造的树上与 NPB 和 AnAnPB 混合独自被组织进二簇和一根树枝。在两棵树上， AAnPB 的一根树枝比 AnAnPB 更近被定位到外集团种类，它含有一些 AAnPB 将比 AnAnPB 和 NPB FPPS 进化历史上更早被分叉。一些 AAnPB 和 NPB 更近位于建议的树和这他们比 AnAnPB 是近亲。在有种系发生的分析的 FPPS 的联合 codon 用法，结果显示那 FPPS 基因和 16S rRNA 基因有类似的进化模式，但是前者似乎在决定在 AAnPB 和他们的亲戚之间的系统发生、进化的关系更可靠、有用。这是第一次尝试在 16S rRNA 基因旁边使用一个分子的标记学习 AAnPB 的发展史，并且学习可能也在在 phototrophic 微生物和光合的基因转移的趋势之中理解进化关系是有用的。

英文摘要：

The study aims to reveal phylogenetic and evolutionary relationship between aerobic anoxygenic phototrophic bacteria（AAnPB） and their relatives,anaerobic anoxygenic phototrophic bacteria（AnAnPB） and nonphototrophic bacteria（NPB,which had high homology of 16S rDNA gene with AAnPB and fell into the same genus）,and validate reliability and usefulness of farnesyl pyrophosphate synthase（FPPS） gene for the phylogenetic determination.FPPS genes with our modified primers and 16S rDNA genes with general primers,were amplified and sequenced or retrieved from GenBank database.In contrast to 16S rDNA gene phylogenetic tree,AAnPB were grouped into two clusters and one branch alone with no intermingling with NPB and AnAnPB in the tree constructed on FPPS.One branch of AAnPB,in both trees,was located closer to outgroup species than AnAnPB,which implicated that some AAnPB would be diverged earlier in FPPS evolutionary history than AnAnPB and NPB.Some AAnPB and NPB were closer located in both trees and this suggested that they were the closer relatives than AnAnPB.Combination codon usage in FPPS with phylogenetic analysis,the results indicates that FPPS gene and 16S rRNA gene have similar evolutionary pattern but the former seems to be more reliable and useful in determining the phylogenic and evolutionary relationship between AAnPB and their relatives.This is the first attempt to use a molecular marker beside 16S rRNA gene for studying the phylogeny of AAnPB,and the study may also be helpful in understanding the evolutionary relationship among phototrophic microbes and the trends of photosynthetic genes transfer.