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基于水淘选分级的长江口最大浑浊带附近颗粒有机碳的来源、分布和保存

ISSN号：0253-4193
期刊名称：海洋学报(中文版)
时间：2015.4
页码：1-15
分类：P736.41[天文地球—海洋地质;天文地球—海洋科学] TQ226.61[化学工程—有机化工]
作者机构：[1]College of Marine Life Science, Ocean University of China, Qingdao 266003, China, [2]Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China, [3]College of Environmental Science and Engineering, Qingdao 266100, China, [4]Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China
相关基金：Foundation item： The National Natural Science Foundation of China under contract No. 40920164004; the National Basic Research Program （973 Program） of China under contract No. 2011CB403602, the Foundation for Innovative Research Groups of the National Natural Science Foundation of China under contract No. 41221004.
相关项目：海洋有机生物地球化学

关键词：表层沉积物, 空间分布, 氨氧化, 微生物群落, 季节, AMOA基因, 基因拷贝数, 东海, ammonia-oxidizing microorganisms, sediment, East China Sea

中文摘要：

氨氧化在海洋的沉积在氮周期起一个重要作用。氧化氨的 archaea (AOA ) 和 betaproteobacteria 的季节、空间的分发(在来自华东海(ECS ) 的表面沉积的 -AOB) 用氨 monooxygenase 子单元(amoA ) 被调查基因。为了描绘 AOA 和 -AOB, 的社区，即时量的聚合酶链反应(qPCR ) 在这研究被执行，与环境参数一起。许多 -AOB amoA 基因(cDNA 的快速的扩大结束的 2.17d (种族) 联系了基因获得三热压力回答的全身的序列：(1 ) 热吃惊蛋白质 70 (OvHSP70 ) ，(2 ) 热吃惊抄写因素(OvHSF ) ，并且(3 ) O 的热吃惊因素绑定蛋白质(OvHSBP ) 。vulgaris。OvHSP70， OvHSF，和 OvHSBP 蛋白质包含了 2 222 bp， 2 264 bp，分别地为 635， 458 和 90 氨基酸编码了的 841 bp。多重顺序排列的结果证明 OvHSP70 的氨基酸序列高度关于另外的种类被保存。同样，謔?謔？？

英文摘要：

Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea （AOA） and betaproteobacteria （13-AOB） in surface sediments from the East China Sea （ECS） were investigated using ammonia monooxygenase ct subunit （amoA） gene. In order to characterize the community of AOA and 13-AOB, real-time quantitative polymerase chain reaction （qPCR） was carried out in this study, along with environmental parameters. The abundance of 13-AOB amoA gene （2.17x 10^6-4.54x10^7 copy numbers per gram wet weight sediment） was always greater than that ofAOA amoA gene （2.18x 105-9.89x 10^6 copy numbers per gram wet weight sediment） in all sampling stations. The qPCR results were correlated with environmental parameters. AOA amoA gene copy numbers in April were positively related to temperature and nitrite concentration （p〈0.05）. 13-AOB amoA gene copy numbers in August correlated negatively with salinity （p〈0.01）, and correlated positively with ammonium concentration （p〈0.05）. With the increase of salinity, the amoA gene copy ratio of AOB to AOA had a tendency to decrease, which suggested 13-AOB dominated in the area of high level ammonium and AOA preferred high salinity area.