中文摘要：

间充质干细胞（mesenchymal stem cells,MSCs）是存在于成体组织间质部分的多能前体细胞,在体外具有自我更新增殖及向成脂、成骨、成软骨分化的潜能,在组织工程和细胞治疗方面具有广阔的应用前景。MSCs在体外长期培养获得足够数量的细胞是MSCs应用的一个重要因素。然而,目前还没有建立MSCs长期传代培养的最适培养体系。该文分别从培养体系中的基础培养基、血清和生长因子对于MSCs细胞长期传代培养的影响进行了论述,旨在为建立MSCs体外长期传代生长的最适培养体系提供理论依据。

英文摘要：

Mesenchymal stem cells（MSCs） are multipotent precursors derived from the stromal fraction of adult tissues. MSCs possess the capacity of self-renewal and multipotency to differentiate into adipocytes, osteoblasts and chondrocytes, which give rise to promising application in tissue engineering and cell therapy. The longterm subculture to harvest high numbers of MSCs is one of the most crucial factors for the application. However, optimal culture system has not been established so far. This paper reviewed the effect of basal medium, serum and growth factor of culture system on MSCs during the long-term subculture respectively, aiming to provide theoretical basis to establish optimal culture system for long-term subculture of MSCs.