中文摘要：

应用RNA干扰技术，建立了RING3 （ really interesting gene3）表达沉默的细胞系，研究了RING3内生水平的表达与其可能的靶基因CyelinA之间的关系．结果表明，在血清饥饿的成纤维细胞NIH／3T3中，用血清刺激后会引起细胞周期蛋白CyelinA的表达；当用RNAi技术使RING3表达抑制后，CyclinA的表达受到相应影响，可见CyclinA的表达受到RING3的调节，其转录激活受到RING3和E2F-1等因子的共同调控，表明RING3与肿瘤发生之间的关系可能与CyclinA的表达有关．

英文摘要：

A pSUPER RNAi system was used to construct a RING3-deficient cell line and research on the relationship between RING3 and Cyclin A. Marked expression of Cyclin A was detected when serumstarved NIH/3T3 stimulated with serum. Once RING3 knocked down, it has a same effects on the Cyclin A expression. The Cyclin A transcription is controlled by both RING3 and E2F-1. The deduction that RING3 relates to tumorigenesis can be partly explained by the expression of Cyclin A.