中文摘要：

以β-actin 为内参基因,采用实时荧光定量聚合酶链式反应（PCR）技术,检测不同时间（12、24、48、96 和192h）不同浓度（0. 01、0. 1、1、10、100、300 μg·L-1）壬基酚（NP）处理下近江牡蛎（Crassostrea hongkongensis）鳃、外套膜和消化腺中HSC70 和HSP70 基因mRNA 的表达水平.结果显示,0. 01 μg·L-1 NP 能诱导近江牡蛎鳃和消化腺中HSC70 和HSP70 基因显著高表达（P〈0. 05）.随着NP 处理浓度的升高,其表达水平逐渐升高,处理一定时间后100 μg·L-1NP 诱导近江牡蛎HSC70 和HSP70 基因表达量显著高于300 μg·L-1 NP 诱导表达量（P 〈0. 05）.HSC70 基因在鳃和消化腺中显著高表达峰值出现在96 h 时,在外套膜中出现在48 h 时;HSP70 基因在鳃中显著高表达峰值出现在48 h 时,在外套膜和消化腺中出现在24 h 时.可见,近江牡蛎HSC70 和HSP70 基因对NP 具有显著反应性.

英文摘要：

With β-actin as reference genes and by means of the real-time fluorescence quantitative RT-PCR method,levels of mRNA expression of HSC70 and HSP70 in gill, mantle and digestive gland of Crassostrea hongkongensis exposedto NP varying in concentration （0. 01, 0. 1, 1, 10, 100 and 300 μg·L-1） were determined at 12, 24, 48, 96 and 192h. Results show that 0. 01 μg·L-1 NP induced significantly expression of HSC70 and HSP70 mRNA in gill and digestivegland（P〈0. 05）. With rising of NP concentration in treatment, the expression level increased gradually. Within a certaintime period, 100 μg·L-1 NP induced significantly a higher expression level than 300 μg·L-1 NP did（P〈0. 05）. Whenthe expression of HSC70 gene in gill and digestive gland peaked at 96 h, it did at 48 h in mantle, and when the expressionof HSP70 in gill peaked at 48 h, it did at 24 h in mantle and digestive gland. It is quite obvious that HSC70 and HSP70genes in the oysters respond significantly to NP.