我国脉红螺自然资源日趋衰减,为制定相应保护措施,需要对脉红螺遗传多样性及遗传结构进行分析研究。本实验利用9个微卫星标记分析了我国沿海脉红螺9个群体的遗传多样性和遗传分化水平。遗传多样性分析结果显示,群体平均等位基因丰度(AR)为8.6~9.5,期望杂合度(HE)为0.705~0.777,观测杂合度(Ho)为0.498~0.626。遗传分化分析结果显示,群体间遗传分化指数(FST)范围为0.012 2~0.093 6,各群体间没有显著的遗传分化,遗传距离(DC)为0.212~0.349。本研究结果表明,我国沿海脉红螺群体具有较高的遗传多样性,各群体间没有显著分化。
In the past decades,the natural resources of Rapana venosa in China have obviously declined because of over-exploitation and habitat destruction. It is very necessary to survey genetic diversity and population structure of R. venosa along the coast of China for the formulation of management policies and the protection of R. venosa resources. In the study,nine polymorphic microsatellite markers were used to evaluate the level of genetic diversity of nine wild R. venosa populations along the coast of China and estimate the degree of genetic differentiation among these populations. The analysis of genetic diversity showed that the mean allelic richness ranged from 8. 6 to 9. 5 and the expected heterozygosity( HE) ranged from 0. 705 to0. 777. The analysis of genetic differentiation showed that the FSTvalues ranged from 0. 012 2 to 0. 093 6 and DCvalues ranged from 0. 212 to 0. 349. It is suggested that the R. venosa possessed high genetic diversity and no clear differentiation was detected between the nine populations.