中文摘要：

目的 观察日本血吸虫（大陆株）硫氧还蛋白重组抗原在小鼠诱导抗血吸虫感染的免疫保护作用。方法 30只雌性C57BL／6小鼠随机分为3组，reSjeTrx免疫组：reSjeTrx（15me／鼠）和ISA720佐剂乳化后采用小鼠相背部多点皮下注射，共免疫3次，间隔2周；ISA720佐剂对照组：小鼠仅注射ISA720佐剂和生理盐水；感染对照组不作任何处理。于末次免疫后3周，各组小鼠经腹部皮肤感染（30±1）条日本血吸虫尾蚴．感染后6周剖杀．门静脉灌注法收集成虫，计成虫数和每克肝组织虫卵数。在免疫前、攻击感染前和小鼠剖杀前分别采血并分离血清。用ELISA检测重组抗原特异性IgG抗体。并对reSjeTrx进行十二烷基磺酸钠．聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹（Western blotting）分析。结果 ELISA检测表明，reSjeTrx免疫组小鼠产生特异性IgG抗体应答，并诱导小鼠产生对攻击感染的减虫率和肝组织减卵率分别为22．8％和29．5％，与ISA720佐剂对照组和感染对照组相比，差异均有统计学意义（P〈0．05）。SDS-PAGE和Western blotting的结果表明，该重组抗原相对分子质量（Mr）约14000（含载体的6个氨基酸）。可被日本血吸虫感染兔血清和reSjcTrx免疫小鼠血清所识别。结论 reSjeTrx免疫小鼠可产生一定的抗血吸虫感染的保护作用。

英文摘要：

Objective To investigate the protective immunity of the recombinant thioredoxin of Schistosoma japonicum（reSjcTrx）in mice. Methods Thirty 6-week old female C57BL/6 mice were randomly divided into 3 groups with 10 each： reSjcTrx with Montanide ISA720 adjuvant, adjuvant control, and infection control. Mice were vaccinated subcutaneously at week 0, 2, 4 with reSjcTrx emulsified in Montanide ISA720 adjuvant. The mice in adjuvant group was injected three times with Montanide ISA720 and saline only. Mice in infection control group were given no injection. Three weeks after final injection, each mouse was challenged with 30±1 cercariae of S. japonicum （Chinese strain）. At the week six after challenge, all mice were sacrificed and perfused. The number of recovered worms and eggs from liver tissue of mice were counted. Sera were collected from mice before immunization, before challenge and before killing. The anti-SjcTrx antibodies in sera were detected with ELISA. Results ELISA showed a high level of specific IgG antibodies in mice immunized with the reSjcTrx. The worm reduction rate and egg reduction rate of reSjcTrx immunization group were 22.8% and 29_5% respectively, significantly higher than those of the control groups （P〈 0.05）. SDS- PAGE and Western blotting revealed that the molecular weight of expressed protein was around Mr 14 000 and could be recognized by sera from rabbit infected with S.japonicum and from mice immunized with reSjcTrx. Conclusion The reSjcTrx induces certain protective immunity against schistosomiasis japonica in mice.