中文摘要：

在自发荧光断层成像（Bioluminescent tomography imaging，BLT）中，双模态融合（光学模态与结构模态）可充分利用结构模态提供的高精度3D几何结构，重建三维表面荧光光通量分布，进而实现小动物内部荧光光源定位．然而，与纯光学模态相比，双模态融合存在采集系统复杂、成本高、数据处理繁琐及存在电离辐射（如CT）等问题．因此，研究基于纯光学3D几何结构的自发荧光光源定位方法对BLT具有重要意义．本文在搭建纯光学自发荧光断层系统（All-optical bioluminescence tomography system，AOBTS）的基础上，提出一种基于多角度光学投影表面重建的三维自发荧光光源定位方法．本方法由基于多角度光学投影的3D表面重建、多角度荧光无缝融合、荧光光通量的量化校正以及自发荧光内部光源重建4部分组成．通过真实小鼠内部植入荧光光源实验表明，与传统纯光学方法相比，本文提出方法不仅改进了3D表面重建方法，而且增加了多角度荧光无缝融合，可实现真实小鼠的三维白发荧光光源定位，初步实验证明具有小动物预临床实验潜力．

英文摘要：

In bioluminescent tomography imaging （BLT）, dual-modality fusion （optical modality and structural modal- ity） can make full use of high accuracy 3D geometrical structures provided by structural modality reconstruct 3D surface light flux distribution and bioluminescence inner light source reconstruction. However, compared with the all-optical modality, dual-modality fusion has the problems of complicated fusion system, high cost compared with all-optical sys- tem, multifarious and exhaustive date processing, and ionizing radiation （for example, CT）. Therefore, the 3D location method of bioluminescence light source based on pure optical 3D geometrical structures has significance for BLT. In this paper, we present a 3D location method of bioluminescence light source based on multi-view projection surface recon- struction, and an all-optical bioluminescence tomography system （AOBTS） is developed for this method. The method consists of 3D surface reconstruction based on multi-view optical projection, multi-view luminescent seamless integration, calibration and quantification of the surface light flux and internal bioluminescence reconstruction. An in-vivo BALB/C mouse with an implanted luminescent light source are used to evaluate the performance of the new method. Compared with the conventional optical methods, the new method improves not only the 3D surface reconstruction method but also the multi-view luminescent seamless integration. It has realized 3D real mouse bioluminescence light source localization, and the preliminary test proves its potential application in clinical trial.