中文摘要：

目的检测分析TCE暴露小鼠肝功能、肝脏Treg细胞分泌的细胞因子IL．10含量以及脾脏Treg细胞数量的变化，探讨Treg细胞与TCE暴露所致肝功能损害的关系。方法雌性BALB／c小鼠饮水摄入2．5mg／ml和5mg／ml的TCE，在2、4、8、12周时采集外周血用全自动生化仪测定肝功能，ELISA检测肝组织匀浆IL．10含量，，流式细胞仪检测脾脏Treg细胞数量。结果与对照组相比，TCE染毒组小鼠AST和ALT水平明显升高，2周和4周时差异有统计学意义（P〈0．05），肝组织匀浆中IL-10含量则明显降低（4周时最低）；脾脏Treg细胞数量也明显降低，2、4、8周时差异均有统计学意义（P〈0．05）；相关分析显示肝功能与肝组织中IL．10含量和Treg细胞数量均呈负相关，且有统计学意义（P〈0．05）。结论TCE暴露引起的肝脏损伤与体内Treg细胞数量减少或功能受损相关。

英文摘要：

Objective To explore the relationships between Treg cell and trichloroethylene exposure induced liver damage through detecting liver function, IL-10 content secreted by hepatic Treg cell and number of spleen Treg cell in mice. Methods Female BALB/c mice were randomly devided into exposed groups that were given 2. 5 mg/ml or 5 mg/ml of TCE （ 10% DMSO solved） contained drinking water, blank group and solvent control group that were only given water or 10% DMSO. At the end of 2, 4, 8 or 12 weeks of exposure, take the blood samples for liver function examination, and the samples of liver and spleen were also taken for detecting IL-10 content in liver by ELISA, and Treg cell number in spleen by flow cytometry. Results Com- pared with control group, levels of AST and ALT in TCE treated groups were all significantly increased at 2 weeks and 4 weeks （ P 〈 0. 05 ）, liver IL-10 content decreased ; and spleen Treg cell number was also significantly decreased at 2,4,8 weeks （P 〈 0.05 ）. Correlation analysis showed that liver function negatively correlated with hepatic IL-10 level and number of spleen Treg cells （P 〈 0.05 ）. Conclusion These results suggested that liver damage induced by TCE exposure is well correlated to the func- tion of hepatic Treg cell and the number of spleen Treg cell.