采用高效液相色谱法测定橄榄果实甲醇提取物中没食子酸的含量,并用二苯基苦基苯肼自由基(DPPH·)法研究了橄榄果实提取物的自由基清除能力。没食子酸质量浓度在8.4.42mg/L范围内,没食子酸峰面积与其进样量呈良好的线性关系(R=0.9993),没食子酸平均质量分数4.03%,相对标准偏差(RSD)(n=6)1.77%,平均回收率98.75%,RSD(n=6)1.21%。该方法简单灵敏,准确可靠,重复性好。橄榄果实甲醇提取物具有较高的自由基清除能力,DPPH·的半数抑制浓度(IC50)为40.14mg/L。
The content of gallic acid in extract of white canarytree (Canarium album (Lour.)Raeusch) fruit was determined by HPLC. The radical scavenging activity of extract was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The linearity of gallic acid content was over the range of 8.4-42 mg/L with good linear correlation ( R = 0.999 3 ). The mean value of gallic acid content was 4.03 %, with relative standard deviation (RSD) ( n = 6) 1.77 %. The average recovery was 98.75 % with RSD ( n =6) 1.21%. The determination method is simple, sensitive, accurate, reliable and with good reproducibility. C. album fruit extract showed a very good DPPH radical scavenging activity ( IC50 ) 40.14 mg/L.