中文摘要：

目的探讨人参皂甙Rh2（G-Rh2）提高人源肝癌细胞HepG2对桦木酸（Bet A）敏感性的作用机制。方法将对数生长期的HepG2细胞分为4组：G-Rh2组（加入7.5μg/ml G-Rh2）、Bet A组（加入10μg/ml Bet A）、G-Rh2＋Bet A组（加入7.5μg/ml G-Rh2和10μg/ml Bet A）及以不加药的细胞作为对照,荧光显微镜观察细胞形态变化,流式细胞术分析细胞凋亡,并检测细胞Caspase-3活性,Western blot检测细胞Caspase-3底物多聚（ADP-核糖）聚合酶[Poly（ADP-ribose）polymerase,PARP]断裂和Caspase-9激活情况。结果与G-Rh2和Bet A组相比,G-Rh2＋Bet A组超过75%的细胞可见凋亡特征,且处于subG1期细胞的比例增加,Caspase-3活性增强,PARP和Caspase-9断裂明显。结论 G-Rh2提高HepG2细胞对Bet A的敏感性是通过细胞凋亡实现的,且涉及线粒体途径。

英文摘要：

Objective To investigate the mechanism of ginsenoside Rh2（G-Rh2） in enhancing the sensitivity of human liver cancer HepG2 cells to betulinic acid（Bet A）.Methods The HepG2 cells in logarithmic growth phase were divided into four groups.The cells in G-Rh2,Bet A and G-Rh2 ＋ Bet A groups were treated with 7.5 μg / ml G-Rh2,10 μg / ml Bet A and 7.5 μg / ml G-Rh2 ＋ 10 μg / ml Bet A respectively,while those in control group were untreated.The cells were observed for morphological change by fluorescent microscopy,for apoptosis by flow cytometry,determined for caspase-3 activity,then analyzed for breakage of poly（ADP-ribose） polymerase（PARP） and activation of caspase-9 by Western blot.Results Compared with those in G-Rh2 and Bet A groups,more than 75% of cells in G-Rh2 ＋ Bet A group showed the characteristics of apoptosis,in which the percentage of cells at subG1 stage and caspase-3 activity increased,while obvious breakages of PARP and caspase-9 were observed.Conclusion G-Rh2 enhanced the sensitivity of HepG2 cells to Bet A through mitochondrion-associated cell apoptosis.