中文摘要：

摘要：为探讨牛膝水煎液和牛膝多糖对体外过氧化氢（H2O2）损伤大鼠肝细胞的影响，通过原位灌流法分离大鼠肝细胞。设不同浓度牛膝水煎液组（6．4、3．2、1．6、0．8mg·mL^-1）和牛膝多糖组（200、100、50、25μg·mL^-1）、H2O2损伤模型组和正常对照组。MTT法测定不同浓度的牛膝水煎液和牛膝多糖对肝细胞的毒性，建立体外H2O2损伤大鼠肝细胞模型并检测肝细胞悬液中AST和ALT活性。结果表明：牛膝水煎液和牛膝多糖均能降低H2O2损伤引起的肝细胞悬液中AST和ALT活性的升高。证明牛膝水煎液及其多糖对H2O2致肝细胞损伤具有保护作用。

英文摘要：

ABSTRACT： To explore the effects of Achyranthese bidentata （Ab） decoction and its polysaccharides （ABPS） on rat hep- atocyte injury induced by hydrogen peroxide （H2O2 ） in vitro, rat hepatocytes were isolated by in situ perfusion in this study. Hepatocytes were divided into different groups treated with Ab decoction （6.4, 3.2, 1.6, 0.8 mg·mL^-1）, ABPS （200, 100, 50, 25μg·mL^-1）, or H2 02 （model group） respectively. The toxicity of Ab and ABPS to the rat hep- atocytes was evaluated by MTT assay. Hepatocyte injury model was established by exploring to H2O2 in vitro, and the activities of ALT and AST in hepatocyte suspension were detected. The results showed that all doses of tested drugs can reduce the activity of AST or AI , hepatocyte suspensions, without significant cytotoxicity. This data suggests both Ab decoction and ABPS have the ctive effects against hepatocyte injury caused by H2O2.