采用麦芽汁液体培养基培养蜜环菌获得菌丝体,采用麦芽汁半固体培养基培养蜜环菌获得蜜环菌菌素、皮壳状菌丝和子实体。以高效液相色谱-二极管阵列检测法(High Performance Liquid Chromatography-Diode Array detector,HPLC—DAD)比较四种菌体的甲醇提取物的成分差异。液体培养的蜜环菌菌丝体与固体培养的蜜环菌菌素、皮壳状菌丝和子实体的蜜环菌素类成分有明显差别。图谱分析表明,菌素与皮壳状菌丝的成分接近,与子实体和液体培养菌丝体之间有较大差别。
Sesquiterpene profiles in methanolic extracts of crustose aerial hyphae, rhizomorphs and fruit bodies of Armillariella mellea grown on semi-solid wort medium, and of fungal mycelium grown in submerged culture on liquid wort medium, were compared using High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD). The sesquiterpene ester components of mycelium grown in submerged culture were distinct from the sesquiterpene esters detected in rhizomorphs, crustose aerial hyphae and fruit bodies. The sesquiterpene ester profile of rhizomorphs was similar to that of crustose aerial hyphae, but was considerably different to the profiles of fungal mycelium grown in submerged culture and fruit bodies.