中文摘要：

基因 nanos 是在果蝇为腹节和细菌行的正常开发要求的母亲的以后的组基因。nanos 相关的基因的表示在另外的税的一个宽广变化与细菌行被联系一。在这研究， 5 ′ - 使方法跑并且在里面克隆方法的 silico 被用来孤立 Bombyx 粗腐殖质 i 的新象 nanos 一样基因，获得的基因与生物信息学工具被分析。通常认为的蛋白质在 Escherichia 关口 i 被表示，抗血清在新西兰白兔子被生产了。结果证明 nanos cDNA 是在完整的长度的 1,913 bp 并且包含开的读物装裱的 954 bp。推出的蛋白质有 317 氨基酸残余，与 35 kDa 的预言的分子量， 5 的等电点。38，并且包含保存 nanos RNA 有约束力的域。推出的蛋白质的保存区域与 nanos 蛋白质分享 73% 相同蜜蜂(蜂 mellifera ) 的保存区域。这基因在就职数字 EF647589 下面在 GenBank 被登记了。nanos 碎片的一个编码顺序成功地在 E 被表示了。关口 i。西方的弄污分析显示那自做的抗血清能明确地检测在原核生物的房间表示的 nanos 蛋白质。

英文摘要：

Gene nanos is a maternal posterior group gene required for normal development of abdominal segments and the germ line in Drosophila. Expression of nanos-related genes is associated with the germ line in a broad variety of other taxa. In this study, the 5＇-RACE method and the in silico cloning method are used to isolate the new nanos-like gene of Bombyx mor/and the gene obtained is analyzed with bioinformatics tools. The putative protein is expressed in Escherichia coli and the antiserum has been produced in New Zealand white rabbits. The result shows that the nanos cDNA is 1,913 bp in full length and contains a 954 bp open reading frame. The deduced protein has 317 amino acid residues, with a predicted molecular weight of 35 kDa, isoelectric point of 5.38, and contains a conserved nanos RNA binding domain. The conserved region of the deduced protein shares 73% homology with the nanos protein conserved region of Honeybee （Apis mellifera）. This gene has been registered in the GenBank under the accession number EF647589. One encoding sequence of the nanos fragment has been successfully expressed in E. coli. Western blotting analysis indicates that homemade antiserum can specifically detect nanos protein expressed in prokaryotic cells.