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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization
  • ISSN号:0513-353X
  • 期刊名称:《园艺学报》
  • 时间:0
  • 分类:Q949[生物学—植物学]
  • 作者机构:[1]Jiangsu Province Key Laboraty of Crop Genetics and Physiology/Key Laboraty of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • 相关基金:Supported by-the National Natural Science Foundation of China (Grant Nos. 30771210, 30500317 and 30600345) and National Basic Research Program of China (Grant No. 2006CB 101701)
中文摘要:

Oryza sativa 和 O。latifolia 分别地属于 Oryza 的 AA 和 CCDD 染色体。在这研究,这些的种间的混血儿种类用胚胎营救技术被获得。混合圆锥花序特点例如长芒,小谷物,一般人所能理解的大紫色耻辱,谷物粉碎和驱散的圆锥花序,类似于父亲的父母,的 O。latifolia 而在是的如此的方面有明显的杂种优势,种高度,到 ering 能力和植物的活力为止。染色体配对和混血儿的 genomic 部件随后在 situ 杂交(GISH ) 用 genomic 被调查并且在 situ 杂交(鱼) 荧光灯分析。基于调查的根尖端的有丝分裂的中期染色体数字,混血儿是一与 36 个染色体三倍性。混血儿的 genomic 体质是 ACD。在 meiotic 中期,我混合花粉溺爱房间,差的染色体配对被识别,大多数染色体是一价的,它在混血儿导致了完全的男绝育。

英文摘要:

Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase Ⅰ of the hybrid pollen mother cell, poor chromosome pairing was identified and most of the chromosomes were univalent, which resulted in complete male sterility in the hybrid.

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期刊信息
  • 《园艺学报》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国园艺学会
  • 主编:杜永臣
  • 地址:北京市中关村南大街12号
  • 邮编:100081
  • 邮箱:ivfyyxb@mail.caas.net.cn
  • 电话:010-68919523
  • 国际标准刊号:ISSN:0513-353X
  • 国内统一刊号:ISSN:11-1924/S
  • 邮发代号:82-471
  • 获奖情况:
  • 第一届全国优秀期刊奖,中国科协第三届优秀期刊奖,第二届国家期刊奖提名奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:43371