中文摘要：

CIPK蛋白激酶家族（CBL-interacting protein kinase）是一类丝氨酸/苏氨酸蛋白激酶家族,与类钙调磷酸酶B亚基CBL（calcineurin B-like protein）蛋白共同形成CBL-CIPK网络,在植物的生长发育和逆境胁迫响应过程中发挥重要作用。烟草中该家族的研究还比较少,本研究从林烟草（Nicotiana sylvestris）中获得一个CIPK家族基因,该基因与拟南芥和杨树中的CIPK3同源性分别为68.4%和87.5%,将其命名为Nsyl CIPK3。氨基酸序列分析表明,Nsyl CIPK3具有CIPK蛋白家族的典型结构特征,在N端和C端分别具有典型的激活环结构域和NAF结构域。进化树分析显示,Nsyl CIPK3属于CIPK蛋白亚家族Ⅱ。表达模式研究表明,该基因在林烟草的叶和腋芽中的表达量相对较高,在主根中的表达量次之,在侧根、茎、花瓣和萼片中的表达量相对较低,并且在烟草成熟期的叶中表达量明显升高。在高盐、紫外光和低钾胁迫下该基因的表达发生不同程度的上调。酵母双杂交结果显示,Nsyl CIPK3可与Nsyl CBL9互作。推测Nsyl CIPK3可能通过与Nsyl CBL9互作形成信号通路,激活下游靶蛋白,参与烟草响应非生物逆境胁迫的信号转导过程。

英文摘要：

CBL-interacting protein kinase（ CIPK） is a kind of serine / threonine protein kinase. By interacting with different calcineurin B-like protein（ CBLs）,CIPKs play important roles in response to various abiotic stresses and the regulation pathway of plant growth and development. Although a lot of work of CBL-CIPK network is finished in Arabidopsis and some other plants,little is known in tobacco model plant. In this study,a CIPK gene NsylCIPK3 was cloned from Nicotiana sylvestris. Sequence analysis indicated that Nsyl CIPK3 showd higher identity with Pt CIPK3 and At CIPK3. Nsyl CIPK3 contained a typical activation loop domain and a highly conserved NAF domain at its N- and C-terminus. The phylogenetic tree indicated that Nsyl CIPK3 belonged to Group II of CIPK family. Expression analysis demonstrated that the expression level of Nsyl CIPK3 was higher in leaves and axillary buds at the vigorous stage and the level in leaves increased evidently in the mature stage of tobacco plants. Real-time quantitative PCR results showed that the expression level of Nsyl CIPK3 increased after high-salt,UV,and low-potassiumtreatments. Yeast two-hybrid assay indicated that Nsyl CIPK3 interacted with Nsyl CBL9. The results demonstrated that Nsyl CIPK3 might be modulated by Nsyl CBL9 and involved in abiotic stress signal transductions pathways in N. sylvestris.