中文摘要：

目的：探讨何首乌苷保护大鼠星形胶质细胞免于奥沙利铂诱导凋亡的作用及可能机制。方法原代培养大鼠星形胶质细胞，并利用流式检测所培养细胞胶质纤维酸性蛋白的表达情况。利用10μmol/L奥沙利铂处理胶质细胞24 h建立凋亡模型，加入浓度梯度的何首乌苷预处理胶质细胞24 h后与奥沙利铂共同作用24 h，台盼蓝染色法观察细胞存活。随后通过Annexin V-FITC/PI双染法流式检测各组凋亡率和检测Caspase-3的活性水平。进而利用JC-1染色法流式检测细胞线粒体膜电位，并利用实时定量RT-PCR与免疫印迹检测BAX与BCL2的mRNA水平和蛋白表达量。结果培养的大鼠星形胶质细胞表达胶质纤维酸性蛋白率达95%以上。奥沙利铂处理诱导凋亡24 h后，对照组胶质细胞凋亡率及Caspase-3活性上升并降低线粒体膜电位，加何首乌苷处理后凋亡率及Caspase-3活性下降而线粒体膜电位上升。相对于对照组，加入何首乌苷处理后BAX基因的mRNA及蛋白表达水平降低，BCL2基因的mRNA及蛋白表达水平上升。结论何首乌苷能恢复凋亡相关基因BAX与BCL2的平衡，保护大鼠皮质星形胶质细胞的线粒体膜电位，并使胶质细胞免于奥沙利铂诱导的凋亡作用。

英文摘要：

Objective To investigate the effect of tetrahydroxy stilbene glycoside （TSG） preventing astrocytes from oxaliplatin induced apoptosis, and relate the mechanism. Methods Primary cultured astrocytes were exposed to 10 μmol/L oxaliplatin for 24 h for inducing apoptosis, and the TSG was added into the culture medium before the oxaliplatin of 24 h. The rate of apoptosis was assayed by Annexin V-FITC/PI staining and flow cytometry analysis, and the Caspase-3 activity were detected by color metric assay. The mitochondrial membrane potential was detected by JC-1 staining and flow cytometry analysis. The BAX and BCL2 expressions were measured by Real-time quantitive polymerase chain reaction （qRT-PCR） for mRNA level and detected by Western blot for protein level. Results The apoptosis rate and Caspase-3 activity had increased on astrocytes induced by oxaliplatin, but TSG can prevent such effects. Furthermore, the mitochondrial membrane potential had decreased on astrocytes induced by oxaliplatin, while TSG can reverse such effects. Moreover, the oxaliplatin could induce the expression of BAX and suppress the expression of BCL2 on mRNA level and protein level, while the TSG had reversed those effects. Conclusions TSG could prevent astrocytes from oxaliplatin induced apoptosis and restore the balance between BAX and BCL2 to protect the mitochondrial membrane potential normal.