中文摘要：

目的：建立HPLC-MS/MS法同时测定山银花中7个有机酸（新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,4-二-O-咖啡酰奎宁酸、3,5-二-O-咖啡酰奎宁酸和4,5-二-O-咖啡酰奎宁酸）的含量,并对其在3种不同植物来源的药材中的含量进行比较。方法：采用Shiseido Capcell Pak-C18色谱柱（4.6 mm×250 mm,5μm）,以0.1%甲酸水溶液（A）-乙腈（B）为流动相,梯度洗脱,流速为1 mL·min^-1,加柱后分流器,使约30%洗脱液进入质谱仪,柱温30℃,进样量5μL;质谱采用ESI-多反应监测（MRM）模式扫描,以水杨酸为内标,脱溶剂温度350℃,脱溶剂气流（N_2）600 L·h^-1,雾化器压力0.2 MPa,毛细管电压4 kV。结果：新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,4-二-O-咖啡酰奎宁酸、3,5-二-O-咖啡酰奎宁酸和4,5-二-O-咖啡酰奎宁酸质量浓度分别在0.032-32.224μg·mL^-1（R^2=0.999 5）、0.168-16.800μg·mL^-1（R^2=0.999 4）、0.038-15.104μg·mL^-1（R^2=0.999 8）、0.010-4.064μg·mL^-1（R^2=0.999 2）、0.013-5.372μg·mL^-1（R^2=0.999 5）、0.079-39.840μg·mL-（-1）（R^2=0.999 5）和0.041-16.208μg·mL^-1（R^2=0.999 3）范围内线性关系良好;定量限（LOQ）为10.0-19.9 ng·mL^-1,平均回收率在94.01%和105.0%之间。样品测定结果显示,山银花中绿原酸和3,5-二-O-咖啡酰奎宁酸含量较高,分别为1.262%-7.408%和0.636%-4.803%;咖啡酸含量较低,为0.003%-0.022%。通过比较不同植物来源的样品,发现其在有机酸的含量和比例方面存在较大差异。结论：本实验建立的方法灵敏、准确,可用于山银花药材中有机酸的含量测定,测定结果可为该药材质量标准的完善提供依据。

英文摘要：

Objective： To establish a method for simultaneous determination of seven organic acids （ neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, eaffeie acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid and 4, 5-di-O-caffeoylquinic acid ） in batches of Lonicerae Flos samples, and to compare their contents in the samples from different origins. Methods： Chromatographic separation was performed on a Shiseido Capcell Pak-C18 column （ 4.6 mm × 250 mm, 5 μm ） at 30℃ of column temperature. 0.1% aqueous formic acid （ A ） and acetonitrile （ B ） were adopted as mobile phase with a gradient elution. The flow rate was set at 1 mL· min^-1, allowing approximately 30% eluent to be injected into the mass spectrometer by a post-column distributor. The injection volume was 5μ L. Detection was carried out on a triple quadrupole mass spectrometer in the negative ion mode using an electrospray source. Multiple reaction monitoring （ MRM ） mode was employed. Salicylic acid was used as internal standard （ IS ）. The operating conditions were as follows ： desolvation temperature, 350℃ ; desolvation gas N2 flow, 600 L· h^-1; nebulizer pressure, 0.2 MPa; capillary voltage, 4 kV. Results： The developed method showed good linearity in the range of 0. 032-32. 224 μg· mL^-1 （ R^2=0. 999 5 ） for neochlorogenic acid, 0. 168-16. 800 μ g· mL^-1（ R^2=0. 999 4 ）for chlorogenic acid, 0. 038-15. 104 μ g· mL^-1 （ R^2=0. 999 8 ）for cryptochlorogenic acid, 0. 010-4. 064 μ g· mL^-1 （ R^2=0. 999 2 ） for caffeic acid, 0. 013-5. 372 μg· mL^-1 （ R^2= 0. 999 5 ） for 3,4-di-O-caffeoylquinic acid, 0. 079-39. 840 μg· mL^-1 （ R2=0. 999 5 ） for 3,5-di-O-caffeoylquinic acid, and 0. 041-16. 208 μg· mL^-1 （ R2=0. 999 3 ） for 4, 5-di-O-caffeoylquinic acid, respectively. The limits of quantification （ LOQs ）ranged from 10.0 to 9.9 ng· mL^-1. The recoveries varied between 94.01% and 105.0%. Chlorogenic acid （ 1. 262%-7. 408% ） and 3, 5-di-O-caffeoylquinic