中文摘要：

以‘洛阳红’牡丹为试验材料,采用RT-PCR方法从其花芽中克隆得到1个开花调控重要转录因子基因FRUITFULL（FUL1）的同源基因,其c DNA开放阅读框长度为768 bp,编码255个氨基酸。序列比对和结构域分析表明,此蛋白具有MADS超家族、K-box超家族的结构域和ARG80多结构域,且具有MADS-box和K-box的稳定保守基序。NCBI blast分析发现克隆得到的氨基酸序列与葡萄中的FUL类蛋白具有较高同源性,为69%。因此将其命名为Ps FUL1,Gen Bank登录号为KX621277。系统进化树分析表明,Ps FUL1与葡萄编码的Vv FUL1亲缘关系最近,属于MADS基因家族中的AP1/FUL亚家族。q RT-PCR表明,Ps FUL1基因在‘洛阳红’牡丹不同组织中表达差异显著,在花芽和花瓣中的表达量最高,苞片和叶片次之,根中最少。在不同品种牡丹（早花‘迎日红’、中花‘洛阳红’、晚花‘花王’）的6个不同开花时期的花瓣中,Ps FUL1基因表达量因花期早晚与开花时期不同而差异显著,表明Ps FUL1基因对牡丹开花及花期的早晚有重要调控作用。

英文摘要：

One important flowering-regulating transcription factor gene FRUITFULL（FUL1） homologous gene was obtained from tree peony（Paeonia suffruticosa ＇Luoyanghong＇） by RT-PCR method.Open reading frame（ORF） length of its cDNA was 768 bp,and could encode 255 amino acids.Sequence alignment and structure domain analysis showed that the protein possessed MADS super family,K-box super family structure domains and multi-domains in ARG80.Also,the protein had stable conservative MADS-box and K-box sequences.NCBI blast analysis found that cloned amino acid sequences had high homology at 69%compared with the FUL-like proteins in Vitis vinifera.This gene was therefore designated as PsFULl and GenBank accession number was KX621277.Phylogenetic analysis showed that it had close relationship with VvFULl in V.vinifera,belonging to subfamily AP1/FUL in MADS gene families.The results of qRT-PCR showed that PsFULl gene displayed significant expression difference in different organs of P.suffruticosa ＇Luoyanghong＇.High expression levels were observed in the bud and petal,followed by bract,leaf and root.Among the six different flowering stages of different varieties of tree peony（early-flowering ＇Yingrihong＇,mid-flowering ＇Luoyanghong＇,late-flowering ＇Huawang＇）,PsFULl gene expression level had significant difference with different flowering stages and blossoms.The results indicated that PsFULl gene had an important regulation effect on the flower formation and blossom in tree peony.