中文摘要：

利用RACE技术从‘陇油6号’油菜中克隆得到1个新的CBF（C-repeat binding factor）基因（GenBank登录号为KP974691）,全长1 000bp,其中包括5′-UTR 114bp,3′-UTR 241bp,开放阅读框645bp,编码215个氨基酸,推测的蛋白质分子量23.8kD,理论等电点为4.86。在氨基酸序列水平上,该基因与多种植物具有较高的一致性,其中与萝卜、紫茎泽兰、卷果涩荠和拟南芥的一致性分别为93.5%、83.7%、81.0%、79.0%。实时荧光定量PCR分析表明,CBF基因在油菜的茎、叶和下胚轴中均有表达,没有组织特异性;该基因表达受低温、ABA、H2O2诱导,MAPKK抑制剂U0126预处理12h再经低温、ABA、H2O2诱导,与单独处理结果相比,CBF基因表达明显降低,表明该基因在油菜适应低温、ABA、H2O2胁迫过程中发挥作用。

英文摘要：

A novel CBFgene was isolated fromBrassica campestris L.Longyou 6by RACE.The full-length cDNA of CBF（GenBank No.KP974691）was 1 000 bp,contained a 5′-UTR of 114 bp,a 3′-UTR of 241 bp,and a 645 bp opening reading frame.The deduced protein was 215 amino acids with molecular weight23.8kDa and isoelectric point 4.86.On the amino acid sequence level,this gene showed high identities with Raphanus sativus,Ageratina adenophora,Malcolmia scorpioides and Arabidopsis thaliana（93.5%,83.7%,81.0% and 79.0%）.RT-PCR analysis revealed that CBF were expressed in the stems,leaves,and hypocotyls with almost no tissue specificity.The transcript level of CBF was increased in response to cold,ABA and H2O2 stress.However,MAPKK inhibitor U0126 pretreatment 12hbefore cold,ABA and H2O2 stress,the expression level of CBF was significantly decreased compared with the single stress.The results suggested that CBFplayed an important role during cold,ABA and H2O2 stress in B.campestris.