中文摘要：

为了优化褪黑素脂质体的生产工艺、提高包封率以及褪黑素的生物利用度，采用响应面法优化超临界CO2制备褪黑素脂质体的工艺参数。在单因素试验基础上以共溶剂浓度、超临界时间、超临界压力为影响因素，脂质体的包埋率为响应值，运用分析法建立了二次多项式数学模型，并分析模型的有效性及各因素间的交互作用。最终响应面法优化的工艺参数为：乙醇浓度为7．5％，超临界温度为50℃，超临界时间为35min，此时包埋率可高达83．2％。采用超临界二氧化碳法制备褪黑素脂质体具有工艺简单、重现性好，且脂质体包封率高、颗粒粒径小、稳定性较高等优点，为今后的实际生产提供了理论基础和科学依据。

英文摘要：

Melatonin is an indole hormone produced by the pineal gland of mammals and humans. It can improve sleep, immune regulation, anti-oxidant and free radical scavenging, anti-tumor, lower blood pressure, regulate heart rate, protection of cardiovascular cells, kidneys, liver and other effects. Melatonin nutrient application in the food industry is greatly limited due to low solubility in water. When melatonin is made into liposomes, it＇s solubility in polar solvent is increased. However, the current preparing methods of melatonin liposomes are complicated, result in the low entrapment rate, high cost, and use too much organic solvent. From 1970s, supercritical fluids technology has been used in liposomal preparation because of its friendliness, nontoxicity to the environment and its solvent-free liposomes and industrial-scale of liposome production. Phospholipid, cholesterol and melatonin can be solvated by supercritical carbon dioxide concurrently with a certain proportion of ethanol under higher pressure. The liposomes can be obtained when the pressure is reduced to release CO2. The homogeneous super-critical solution is expanded and simultaneously mixed with the aqueous phase to yield liposomes encapsulating the lipid soluble drugs under the lower pressure. The experiment was conducted to enhance the bioavailability of melatonin by improving entrapment rate in this study. The response surface methodology was used to optimize the technical conditions of supercritical carbon dioxide method for the preparation of melatonin liposome. The entrapment rate of melatonin liposome as a major index was optimized based on the ethanol concentration, supercritical temperature and time by response surface methodology using single factor experiments. The applicability of the quadratic-multinomial-mathematical model developed based on the response surface methodology was verified. The results indicated that the highest entrapment rate of melatonin liposome was reached to 83.2% with the supercritical time of 35 min, the supercrit