中文摘要：

本研究考察了氧化低密度脂蛋白（α-LDL）刺激的U937细胞致炎细胞因子白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、抗炎细胞因子白细胞介素10（IL-10）及其受体（IL-10R）的蛋白及mRNA的表达，同时观察银杏叶提取物（GbE）对它们的作用。U937细胞用100mg·L^-1 ox-LDL刺激24h形成泡沫细胞，同时分别加入不同浓度的GbE（0．1，1及10μg·L^-1）共孵育，采用酶联免疫吸附试验（ELISA）及逆转录聚合酶链式反应（RT-PCR）方法检测IL-1β、TNF—α、IL-10和IL-10R的蛋白或mRNA表达。U937泡沫细胞组IL-1β、TNF—α、IL-10和IL-10R的蛋白或mRNA的表达较对照组显著增加（P〈0．01）。GbE组IL-1β及TNF—α的蛋白和mRNA的表达水平明显降低，IL-10的蛋白、IL-10和IL—10R的mRNA表达水平明显提高，与U937泡沫细胞组相比差异显著（P〈0．05，P〈0．01）。GbE对U937泡沫细胞致炎细胞因子IL-1β及TNF-1β表达的显著抑制作用，对抗炎细胞因子IL-10及其受体IL-10R表达的显著上调作用可能是其抗atherosclerosis（As）的机制之一。

英文摘要：

This study is to investigate the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in U937 foam cells and effects of Ginkgo biloba extract （GbE） on the cytokines. U937 cells were cultured with different concentrations of GbE （0. 1, 1, and 10μg·L^-1） , and stimulated by 100 mg · L^-1 oxidized low density lipoprotein （ox-LDL） for 24 h. The expressions of interleukin-1β （IL-1β） , tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） in culture solution were detected by enzyme-linked immunosorbant assay （ELISA） and reverse transcriptase polymerase chain reaction （RT-PCR）. The results showed that incubated with 100 mg·L^-1 ox-LDL for 24 h, the U937 cells became foam cells, the protein or mRNA expressions of IL-1β, TNF-α, IL-10, and its receptor IL-10R in U937 foam cells were higher markedly than those in normal U937 cells. When the cells were pretreated with GbE （0. 1, 1, and 10μg· L^-1 ）, the increases of IL-1β and TNF-α in U937 foam cells were remarkably inhibited, but IL-10 expression increased greatly. Especially when cells were pretreated with 10μg · L^-1 GbE, the protein and mRNA expressions of IL-1β and TNF-α were markedly lower than those in U937 foam cells. The protein expression of IL-10 and mRNA expressions of IL-10 and its receptor IL-10R were markedly higher than those in U937 foam cells. GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α, but up-regulated the production of anti-inflammatory cytokine IL-10 and its receptor IL-10R in U937 foam cells, which might be related with its anti-atherosclerotic actions.