中文摘要：

该研究旨在探讨重组MS100A6蛋白对乳腺癌细胞株MCF．7的增殖、凋亡、迁移及侵袭能力的影响。利用原核表达制备重组．＆S100A6蛋白（GSTohS100A6），SDS．PAGE显示其大小为36kDa，Westernblot显示其可以被S100A6抗体特异识别，BCA法测定1L菌液共收获约16．7mg蛋白；将其作用于人乳腺癌细胞MCF-7，MTT显示细胞培养48h时，浓度为100μg／mL和300gg／mL的GST-hS100A6组的D492值较GST组增加29．1％和84．6％（P〈0．05），提示S100A6促进MCF．7细胞增殖；平板克隆形成实验显示GST-hS100A6组的克隆形成率较GST组高38．7％（P〈0．05），提示S100A6促进MCF．7的克隆形成；Hoechst染色显示GST-hS100A6组在24h时细胞凋亡率较GST组减少67．8％（P〈0．05），48h时细胞凋亡率较GST组减少58．4％（p〈0．05），提示S100A6抑制MCF-7细胞凋亡：划痕实验显示在24h时GST-hS100A6组的划痕愈合率为GST组的2．2倍（P〈0．05），提示S100A6促进MCF-7细胞迁移；Transwell显示GST-hS100A6组在24h时穿膜细胞数较GST组增加88．1％（P〈0．05）。提示S100A6促进MCF-7细胞侵袭。以上结果显示S100A6对人乳腺癌具有一定的促进作用，有可能成为乳腺癌分子诊断的标志物和治疗的新靶标。

英文摘要：

To aim at effects of human S 100A6 on proliferation, apoptosis, migration and invasion of human breast cancer cell line MCF-7, recombinant protein GST-hS 100A6 was purified from bacteria BL21 that identi- fied as 36 kDa by SDS-PAGE and recognized by S100A6 antibody with Western blot. MCF-7 were treated by GST- hS 100A6 with different concentrations while GST as a control group. MTT was used to detect the cell proliferation. Results showed that after 48 h, the D492 value of 100 μg/mL and 300 p.g/mL group of GST-hS100A6 increased by 29.1% and 84.6% compared with GST group, respectively （P〈0.05）. Colony-forming assay showed that the abil- ity of colony formation of GST-hS100A6 increased by 38.7% compared with GST group （P〈0.05）. Hoechst stain- ing showed that cell apoptosis rate of GST-hS100A6 group decreased by 67.8% compared with GST group at 24 h （P〈0.05） and decreased by 58.4% at 48 h （P〈0.05）. Results of wound healing assay indicated that the healing rate of GST-hS100A6 group was 2.2 times of GST group （P〈0.05） at 24 h. Transwell invasion assay showed that the trans- membrane cell number of GST-hS 100A6 group increased by 88.1% （P〈0.05） compared with GST group at 24 h. To sum up, S 100A6 could promote cell proliferation, colony formation, migration, and invasion, but inhibit cell apop- tosis on human breast cancer cell line MCF-7, indicates that S100A6 has a promoting effect on human breast cancer which would be a new molecular target for treatment of human breast cancer.