中文摘要：

目的：采用高效液相色谱法建立红蓼乙酸乙酯部位的指纹图谱。方法：以Agilent C18（4.6 mm×250 mm,5μm）为色谱柱;DAD检测器;流动相为甲醇-水梯度洗脱,流速1.0 mL/min,柱温30℃。结果：以26个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间RSD均小于3%,10批不同产地红蓼相似度大于0.9。结论：此方法精密度、稳定性和重复性良好,有助于红蓼药材的质量控制。

英文摘要：

Objective：To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale.Methods：Agilent C18（4.6 mm×250 mm,5 μm）;DAD detector;Mobile phase：methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min,and at a column temperature of 30 ℃.Results： 26 peaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values were less than 3% in the precision and repeated test.Conclusion： The repetition,stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.