中文摘要：

建立高效液相色谱-串联质谱法（HPLC-MS/MS）同时检测食品中所含碱性嫩黄O、酸性橙II和酸性金黄的检测方法。样品用经甲醇-50 mmol/L乙酸铵洗脱,经高效液相色谱-串联质谱法测定。结果表明3种组分在0.10-2.00 mg/L呈良好的线性关系,相关系数在0.999 3以上。样品回收率81.3%-110.5%,相对标准偏差（n=3）为2.0%-5.4%,定量限在1.0 mg/kg以下。该方法操作简单、灵敏度高、结果准确可靠,可应用于食品中3种偶氮类工业染料的定量及确证分析。

英文摘要：

To develop a method for the simultaneous determination of auramine O,orange acid Ⅱ and metal yellow with HPLC- MS/MS.Samples was eluted by methanol and 50 mmol/L ammonium,and determined by HPLC- MS/MS.The calibration curve of the method was linear over the range of 0.10 to 2.00 mg/L with a correlation coefficient of 0.9993.The recoveries were in the range of 81.3% to 110.5% with relative standard deviations of 2.0%to 5.4%.The limit of quantification（ LOQ） was under 1.0mg/kg.The method is sensitive,accurate and can be used for seasoning illegally added auramine O,orange acidⅡ and metal yellow of the simultaneous determination.