中文摘要：

为了揭示烟草内生细菌多样性,选择K326、云85和红花大金元3个烤烟品种进行内生细菌的分离和16S rDNA序列分析鉴定,获得不同品种烟草内生细菌的多样性特征.结果显示,27株内生细菌分别属于枯草芽孢杆菌Bacillus subtilis（33％）、蕈状芽孢杆菌Bacillus mycoides（4％）、芽孢杆菌属Bacillussp.（1 1％）、肠杆菌属Enterobacter sp.（4％）、寡养单胞菌Stenotrophomonas sp.（4％）、热带根瘤菌Rhizobiumtropici（4％）、假单胞菌属Pseudomonas sp.（4％）、土地芽孢杆菌属Terribacillus sp.（4％）,芽孢杆菌属Bacillus sp.为优势细菌类群.3个品种的Shannon index（H＆#39;）以K326最高、为2.02,其次是云85、为1.48,红花大金元最低、为1.3.

英文摘要：

The diversity of endophytic bacteria in tobacco tissues originating from three flue-cured tobacco varieties （ K326, YUN 85 and Honghuadajinyuan ） in Guizhou province was surveyed, to describe the distribution of isolated endophyte species in different varieties, and to assess their specificity and abundance. Endophytic bacteria were isolated from surface-sterilized tissues, and the strains were identified based on molecular characteristics of analysis of the 16S rDNA sequence. 27 strains belonged to eight different taxa, they were Bacillus subtilis （ 33% ） , Bacillus mycoides （ 4% ） , Bacillus sp. （ 11% ） , Enterobacter sp. （ 4% ） , Stenotrophomonas sp. （ 4% ） , Rhizobium tropici （ 4% ） , Pseudomonas sp. （ 4% ） , Terribacillus sp. （ 4% ） , dominated by Bacillus sp. Shannon Wiener diversity index of K326, YUN 85 and Honghuadajinyuan （ HD ） were 2.02, 1.48 and 1.3 respectively.