中文摘要：

为建立鱼藤酮免疫分析方法，以鱼藤酮为原料合成4-{2-[（2R，6as，12as）-1，2，6，6a，12，12a-六羟基-2-异丙烯基-8，9-二甲氧基色烯（3，4-b）喃（2，3-h）色烯-6-亚基]肼基}-4-丁酸（简称鱼藤酮腙琥珀酸半酯，RH-HS）半抗原，通过碳化二亚胺法（EDC）、活酯法（AE）将半抗原与牛血清蛋白（BSA）偶联制备免疫原Ⅰ、Ⅱ。将免疫原分别免疫BALB／c鼠获得相应的抗体Ⅰ和抗体Ⅱ，测定抗体冻干粉效价，分别为4．10×10^5和8．29×10^5。并进行抗原抗体的竞争抑制能力、特异性和鱼藤酮的添加回收试验。结果表明：抗体Ⅰ、Ⅱ对鱼藤酮最低检测限（IC20）分别为0．440μg／mL和0．437μg／mL。两抗体对吡蚜酮、克百威的交叉反应率均小于1．1％，抗体I对4个与鱼藤酮结构相类似化合物的交叉反应率均高于56％，较抗体Ⅱ高。鱼藤酮的免疫分析技术（ELISA）与HPLC检测手段相比，在检测线性范围和灵敏度方面，两者差异不明显；在样品前处理方面，ELISA显示出快速、简单、有效的优点。

英文摘要：

In order to establish the immunoassay method of rotenone, the hapten 4-{ 2-[ （2R, 6as, 12as）- 1, 2, 6, 6a, 12, 12a-hexahydro-2-isopropenyl-8, 9-dimethoxychromeno （3,4-b） furo （2, 3-h） chromen-6-ylidene ] hydrazinyl } -4-oxobutanoic acid （RH-HS） was synthesized from rotenone. The hapten was conjugated to bovine serum albumin （BSA） , with the 1-ethyl-3-3-dimethylaminopropyl carbodiimide （EDC） method and active ester （AE） method to prepare immuneogen Ⅰ and immuneogen Ⅱ. Corres- ponding antibodies were prepared through the immuneogens in BALB/c mouse. Titers of antibody Ⅰ and antibody Ⅱ were 4.10 × 10^5 and 8.29 × 10^5, respectively. With the experiments about the affinity, spe- cificity of antibodies and the recoveries of rotenone, the results showed that the limit of detection （IC20） for rotenone were 0. 440 μg/mL and 0. 437 μg/mL. The percentages of cross-activity to pymetrozine and carbofuran were less than 1.1% , and for the other 4 similar compounds, the antibody I was much higher than antibody U with the percentage of 56% above. The ELISA which was established by the antibody Ⅰ , was content with detecting the similar compounds of rotenone. There was not diversity to compare with HPLC in lineal range and sensitivity. But they had displayed a rapid, simple, effective method in sample preparation.