中文摘要：

目的对已建立的慢性阻塞性肺疾病（COPD）寒饮蕴肺证大鼠模型的可信性作出评价。方法建立大鼠COPD模型与COPD寒饮蕴肺证模型,观察各给药组动物一般情况,肺功能测定、血清中白细胞介素-8（IL-8）与白细胞介素-10（IL-10）及病理组织的变化。结果COPD寒饮蕴肺证大鼠模型的一般情况与寒饮蕴肺证的临床表现相符,各项指标的变化（除IL-8和IL-10外）也与COPD的指标变化相一致：大鼠体重减轻,吸气阻力和呼气阻力升高、肺顺应性下降,但IL-8升高和IL-10浓度与正常组相比无显著性变化（P〉0.05）,病理切片显示肺组织出现炎性损伤。在撤除造模因素4w后,模型大鼠的一般情况及各项指标没有明显好转。用温阳化饮方和布地奈德干预的模型大鼠症状好转,肺功能改善,IL-8和IL-10浓度与模型大鼠相比无显著性变化（P〉0.05）,病理切片示炎性损伤明显减轻。结论用本实验方法建立COPD寒饮蕴肺证大鼠模型是可行的。

英文摘要：

Objective To assess the credibility of the established rat model of accumulation of cold and harmful fluid in lung with chronic obstructive pulmonary disease （COPD）. Methods Randomization, comparison and animal modeling were adopted. Results The general situations of rat model of accumulation of cold and harmful fluid in lung of COPD were in accordance with clinical manifestations of accumulation of cold and harmful fluid in lung and the changes in indexes（except IL-8 and IL-10）were basically coincided with those of COPD. Body mass of rat was reduced,inspiratory resistance（Ri）and expiratory resistance（Re）in- creased,and compliance of lung（CL）reduced. But the concentrations of serum IL-8 and IL-10 did not present significant difference compared with normal group（P〉0.05）. Pathological section indicated inflammatory damage of pulmonary tissue. 4 weeks after withdrawal of modeling factors, the general situations and every index of modeled rats did not present apparent improvement. For modeled rats with the intervention of Wen Yang Hua Yin Fang （formula for warming yang and resolving harmful fluid）, their symptoms were released,lung functions improved,and no significant changes presented in serum IL-8 and IL-10 concentrations compared with modeled rats（P〉0.05）. Inflammatory damage indicated in the pathological section was alleviated apparently. Condusion It is feasible to set up the rat models of accumulation of cold and harmful fluid in lung of COPD.