目的 研究860 MHz微波辐射对小鼠场景性条件恐惧(context conditioned fear)形成和消退的影响.方法 用频率为860 MHz、功率密度分别为380μW/cm2、550μW/cm2的微波辐照小鼠.辐照时间分别设定为30min和2h,因此共分成5组,每组15只动物.在暗箱内用75 V电压电击动物足部的方法 产生与之关联的场景性条件恐惧,而后观察辐照后小鼠在特定时间内出现僵立(Freezing)的时间和次数.结果 足部电击后24h时的僵立时间的值:对照组为(2.13±4.17)s,微波辐照2h的两个组分别为(3.93±6.99)s和(2.47±3.34)s,Nemenyi的检验(P=0.004)结果 :对照组为32.63333,而微波辐照2h的两个组分别为52.46667和39.76667;僵立次数的数值:对照组为(0.73±1.16)次,微波辐照2 h的2个组分别为(0.86±1.41)次和(1.07±1.16)次,Nemenyi检验(P=0.014)结果 :对照组为33.26667,而微波辐照2h的2个组分别为50.76667和40.90000.结论 经过相对较长时间的860MHz微波辐照的小鼠表现为对于特定的场景产生更稳固的恐惧,且难以消退.
Objective To investigate the effects of 860MHz microwave on the formation and extinction of context conditioned fear in mice. Methods The mice were exposed to 860MHz continuous microwave ( power density were 380 μW/cm2 or 550 μW/cm2, respectively) for 30 min or 2 h, which then were divided in to 5 groups.Each group consisted of 15 animals. Footshocks were used to induce context conditioned fear by 75 voltages. The frequency and time of freezing after irradiation were investigated. Results When 24 h after foot shocking, the values of freezing time: control group was 2.31 ±4. 17 , two groups of the microwave irradiation 2 h were 3.93 ±6.99 and 2.47 ± 3.34, the Nemenyi test results (P = 0.004): control group was 32.63333, while two groups of the microwave irradiation 2 h were 52.46667 and 39.76667; and the values of freezing frequency: control group was 0.73 ± 1.16 , two groups of the microwave irradiation 2 h were 0.86 ± 1.41 and 1.07 ± 1.16, the Nemenyi test results of (P=0. 014): control group was 33. 26667, while two groups of the microwave irradiation 2 h were 50. 76667 and 40.90000. Conclusion The mice receiving relatively longer period of microwave irradiation showed more stable memory of the context conditioned fear.