中文摘要：

目的：观察转录因子Foxm1在慢性鼻-鼻窦炎（CRS）患者鼻窦黏膜中的表达与分布,及其与黏蛋白MUC5AC的关系。方法：取伴或不伴鼻息肉的CRS患者鼻窦黏膜各25例,另外取15例正常鼻腔黏膜作为对照组,用苏木精-伊红染色、糖原过碘酸雪夫染色（PAS染色）、免疫组织化学技术及实时荧光定量PCR（qRT-PCR）检测Foxm1及MUC5AC在各组鼻窦黏膜中的表达情况,同时统计伴或不伴息肉CRS中Foxm1与MUC5AC表达间的相关性。结果：伴或不伴鼻息肉CRS患者鼻窦黏膜主要的病理特征是杯状细胞、炎症细胞及黏膜下腺体增生。免疫组织化学结果显示：Foxm1、MUC5AC主要表达于伴或不伴鼻息肉的CRS黏膜上皮内,较对照组明显增多,差异有统计学意义（P〈0.05）。qRT-PCR示,Foxm1及MUC5AC在CRS组织中的表达较正常鼻窦黏膜上皮显著增高（P〈0.05）,而在伴或不伴鼻息肉的CRS组织之间差异无统计学意义（P〉0.05）。伴或不伴鼻息肉的CRS中Foxm1与MUC5AC的表达呈显著正相关（P〈0.05）。结论：在伴或不伴鼻息肉的CRS中Foxm1的表达均上调,表明转录因子Foxm1在CRS黏蛋白分泌中可能具有重要的作用。

英文摘要：

Objective：To explore the expression of Forkhead box protein m1（Foxml）in the sinus mucosa of patients with chronic rhinosinusitis（CRS）and the relationship between Foxm1 and MUC5AC for further understanding of the pathogenesis of CRS.Method：We obtained the sinus mucosa from 25 CRS with polyps（CRSwNP）patients and 25 CRS without polyps（CRSsNP）patients as two experimental groups.Nasal mucosa from 15 normol cases were obtained as control group.We used HE,Periodic acid-schiff staining to observe the histopathological change of each sample.The expression of Foxm1 and MUC5AC were determined by immunohistochemistry（IHC）and qRT-PCR in each group.We also analysed the relationship between Foxm1 and MUC5AC.Result：The HE and PAS staining showed that in the CRSwNP and CRSsNP patients,the main histopathological features was the hyperplasia of goblet cell,inflammatory cell and submucosal gland.IHC showed that Foxm1 and MUC5AC expression were higher in CRSwNP and CRSsNP compared with normal sinus mucosa.qRT-PCR also revealed that Foxm1 and MUC5AC expression was higher in CRSwNP and CRSsNP than in normal mucosa（P〈0.05）.Foxm1 and MUC5AC had a significant positive correlation.Conclusion：The expressions of Foxm1 and MUC5AC were increased in CRS,and Foxm1 may play an important role in the process of hypersecretion of MUC5 AC in CRS.