中文摘要：

目的：用蛋白质组技术分析顺铂（cisplatin，DDP）作用于肝癌SMMC-7721细胞后细胞内蛋白质组的变化。方法：采用四氮唑蓝还原反应法（MTT）测定细胞生长抑制率。顺铂（10mg·L^-1）作用肝癌SMMC-7721细胞24h，分别收集顺铂处理组与对照组细胞，提取细胞内总蛋白并采用双向电泳（2-DE）分离，Image Master 2D Platinum软件分析顺铂处理前后差异表达蛋白质，切取明显差异点，基质辅助激光解吸飞行时间质谱（MALDI—TOF-MS）鉴定差异蛋白。结果：顺铂可抑制肝癌SMMC-7721细胞的生长，其抑制作用具有剂量及时间依赖性。质谱鉴定出4个已知序列的差异蛋白点。结论：顺铂处理后肝癌SMMC-7721细胞内差异表达的蛋白可能与顺铂的抗肿瘤作用机制有关。

英文摘要：

OBJECTIVE To study changes of protein expression in SMMC-7721 cells administered with cisplatin （DDP） using a comparative proteomic approach. METItODS Inhibitory effects of DDP on SMMC-7721 cell line were measured by MTT test. SMMC-7721 cells were collected after being exposed to DDP （10 mg.L^-1 ） for 24 hours, then total proteins were extracted and separated by two dimensional electrophoresis（2-DE）. Spots with dominant different expression were selected by Image Master 2D Platinum software analysis and identified with matrix assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. RESULTS DDP induced SMMC-7721 cell death was in a time and dosage dependent manner. Four different expression protein spots were identified by MALDI-TOF-MS. CONCLUSION These altered expression proteins might be related to the apoptotic mechanism of DDP-treated SMMC-7721 cells.