中文摘要：

【目的】开发可用于植物遗传多样性分析和遗传图谱构建的新型标记。【方法】根据植物结构基因内含子拼接位点的保守序列，设计扩增内含子的内含子-外显子拼接位点（intron targeted intron-exon splice junction，IT-ISJ）标记引物，并用于陆地棉遗传图谱构建。【结果】利用704个IT-ISJ引物组合，对陆地棉高品质品种渝棉1号和多显性基因标记系T586进行多态性筛选，获得49个多态性引物组合，占筛选引物的7．0％。49个多态性引物组合在（渝棉1号×T586）F2：7，重组近交系群体270个系中检测到58个位点。以58个IT-ISJ、150个SSR和8个形态标记进行连锁分析，构建的遗传连锁图包括22个连锁群、113个位点（49个IT-ISJ、62个SSR和2个形态标记）。连锁图覆盖714．5cM，占棉花基因组的16．1％，标记间平均长度为6．3cM。【结论】IT-ISJ标记多态性高，可有效地用于陆地棉遗传连锁图谱的构建。

英文摘要：

[Objective] To develop a new DNA marker, which could be used in genetic diversity analysis and genetic map construction in plant. [Method] IT-ISJ （intron targeted intron-exon splice junction） marker primers designed according to the intron-exon splice junction conserved sequences were used to construct cotton genetic linkage map. [ Result ] Fourty-nine out of 704 IT-ISJ primer combinations showed polymorphism between high quality upland cotton cultivar Yumian 1 and multiple dominant gene line T586, and the polymorphic primer combinations accounted for 7.0% of total primer combinations. Fourty-nine IT-ISJ primer combinations were used to genotype 270 F2：7 recombinant inbred lines developed from （Yumian 1×T586） F2 and 58 IT-ISJ lbci were obtained. Fifty-eight IT-ISJ, together with 150 SSR and 8 morphological loci, were used to conduct linkage analysis, and a linkage map including 22 linkage groups and 113 loci （49 IT-ISJ, 62 SSR, and 2 morphological loci） was constructed. The linkage map covered 714.5 cM, accounting for 16.1% of cotton genome, with an average interval of 6.3 cM between two markers.[ Conclusion ] The present study demonstrated that IT-ISJ marker is highly polymorphic, and it could be effectively applied in plant genetic map construction.