中文摘要：

目的克隆鉴定白纹伊蚊嗅觉受体基因OR7,并对其表达谱和功能进行分析。方法提取成蚊总RNA,采用RT-PCR技术扩增白纹伊蚊嗅觉受体基因OR7的全长编码基因,并检测OR7在不同虫期和不同组织器官中的表达谱;将OR7基因克隆于真核表达载体pME18s中,转染HEK293细胞,利用钙成像技术初步分析其对气味分子刺激的钙调功能。结果成功克隆出白纹伊蚊嗅觉受体基因OR7的全长编码基因,经测序得知其编码区序列长度为1395 bp;表达谱分析显示,其在蚊幼虫、蛹、成蚊中都有表达,且在雌蚊嗅器中表达显著。钙成像结果显示OR7对气味分子刺激后的钙离子通道有一定的调节功能。结论克隆出白纹伊蚊的嗅觉受体基因OR7,初步分析显示其在雌蚊中显著表达,并具有对气味分子的识别功能。

英文摘要：

Objective To clone and identify olfactory receptor odorant receptor 7（OR7） gene of Aedes albopictus and analyze its expression profile and calcium regulation function. Method RT-PCR was used to amplify the olfactory receptor OR7 gene of Ae. albopictus and OR7 expression was detected in different tissues and organs. The coding sequence of OR7 gene was cloned in eukaryotic expression vector pME18 s, which was then transfected into HEK293 cells. The calcium callback function in response to odor molecule stimulation was analyzed by calcium imaging technique. Results The OR7 gene of Ae. albopictus was cloned and sequence analysis showed that its coding region was 1395 bp. RT-PCR detected OR7 expression in the larvae,pupae and adult mosquitoes, especially in female mosquitos. Preliminary analysis of calcium callback function demonstrated the specific regulation of calcium absorption by OR7 in response to odor molecule stimulation. Conclusion The OR7 gene of Ae. albopictus has been cloned successfully. OR7 is highly expressed in female mosquitos and is capable of specific recognition of the odor molecules.